Taq DNA Polymerase without Mg2+
PCR
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- Species
- Thermus aquaticus
- Application
- Polymerase Chain Reaction (PCR)
- Characteristics
- Taq DNA Polymerase is a thermostable DNA polymerase isolated from an E. coli strain that carries the Taq DNA polymerase gene. Taq DNA polymerase is the most common polymerase used for PCR .
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- Application Notes
- The applications of Taq DNA Polymerase include the following: PCR* 3' A-tailing of blunt ends Primer extension DNA sequencing.
- Comment
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Terminal transferase activity: Taq DNA Polymerase has terminal transferase activity, which results in the addition of a single nucleotide (adenosine) at the 3' end of the extension product.
High purity: No contamination activity has been detected in standard test reactions.
Unit Definition: One unit is the amount of enzyme that can incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C. - Restrictions
- For Research Use only
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- Buffer
- 500 mM KCl, 100 mM Tris HCl (pH 9.0 at 25°C), 1% Triton X-100 Buffer. This buffer is optimized for use with 200 µM dNTPs. Note: If the reaction is performed without this buffer, then add 0.1% Triton X-100 (final concentration) to ensure high activity.
- Storage
- -20 °C
- Storage Comment
- Store the product at -20°C. The enzyme can be shipped at room temperature or stored at 37°C for seven days without any significant loss of activity.
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