AflII
RD
20000 U/mL
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- Application
- Restriction Digest (RD)
- Characteristics
- An E. coli strain that carries the AflII gene from Anabaena flos-aquae (CCAP 1403/13f). The AflII enzyme recognizes and cuts the sequence C^TTAAG.
- Components
- AflII enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
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- Comment
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- Restriction enzyme digestion of doublestranded DNA
- Reaction: 1X Universal Restriction Enzyme Reaction Buffer
- Reaction Buffer: Buffer 1: 50%, Buffer 2: 100%, Buffer 3: 10%, Universal Buffer: 100%
- Heat Inactivation: 65°C for 20 min
- Methylation Sensitivity:
- dam methylation: Not Sensitive
- dcm methylation: Not Sensitive
- CpG Methylation: Not Sensitive
- Restrictions
- For Research Use only
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- Concentration
- 20000 U/mL
- Buffer
- 10 mM Tris-HCl ( pH 7.5), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, and 50 % (v/v) Glycerol
- Storage
- -20 °C
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