Bst DNA Polymerase, Large Fragment
DNA Man
8 U/μL
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- Application
- DNA Manipulation (DNA Man)
- Characteristics
- Bst DNA Polymerase, Large Fragment is a DNA polymerase from Bacillus stearothermophilus that is modified so that it retains its 5'→ 3' DNA polymerase activity while lacking the intrinsic 5'→ 3' exonuclease domain. This polymerase also has strand displacement capabilities making it an ideal candidate for isothermal amplification.
- Components
- Enzyme supplied with 2X Reaction Buffer
- Unit Definition
- One unit is defined as the amount of Bst DNA Polymerase, Large Fragment that that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 65°C.
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- Comment
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- Isothermal amplification
- DNA sequencing of high GC DNA
- Sequencing from very low amounts of template DNA
- DNA strand displacement amplification
- Restrictions
- For Research Use only
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- Concentration
- 8 U/μL
- Buffer
- 10 mM Tris-HCl ( pH 7.5), 0.1 mM EDTA, 1 mM DTT, 50 mM KCl, 0.1 % Triton® X-100 and 50 % (v/v) Glycerol.
- Storage
- -20 °C
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