DpnI
RD
20 U/μL
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- Application
- Restriction Digest (RD)
- Characteristics
- The DpnI restriction enzyme digests DNA at GmeATC sites, requiring N6-methylation of the adenine residue for activity. DNA purified from a dam+ strain will be a substrate for DpnI due to the adenine methylation. DpnI cleaves hemi-methylated dam sites 60 X more slowly than fully methylated.
- Components
- Enzyme supplied with 10X Reaction Buffer
- Unit Definition
- One unit is defined as the amount of Dpn1 required to digest 1 μg of dam methylated pBR322 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
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- Comment
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- Molecular cloning
- Site directed mutagenesis
- Restriction site mapping
- Genotyping
- Southern Blot
- SNP
- Restriction fragment length polymorphism (RFLP)
- Restrictions
- For Research Use only
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- Concentration
- 20 U/μL
- Buffer
- 10 mM Tris-HCl ( pH 7.5), 300 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 500 μg/mL BSA, and 50 % (v/v) Glycerol.
- Storage
- -20 °C
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