KpnI ClearCut
RD
20000 U/mL
-
- Application
- Restriction Digest (RD)
- Characteristics
- ClearCut Enzymes are engineered to have high specificity (reduced star activity) and high activity (cuts in 5-15 minutes).
- Components
- KpnI ClearCut enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
-
-
- Comment
-
- Restriction enzyme digestion of doublestranded DNA
- Reaction: 1X Universal Restriction Enzyme Reaction Buffer. Incubate at 37°C
- Source: An E. coli strain that carries the KpnI gene from Klebsiella pneumoniae OK8 (ATCC 49790). The KpnI ClearCut enzyme recognizes and cuts the sequence GGTAC^C.
- Reaction Buffer: Buffer 1: 100%, Buffer 2: 25%, Buffer 3: 10%, Universal Buffer: 100%
- Methylation Sensitivity
- dam methylation: Not Sensitive
- dcm methylation: Not Sensitive
- CpG Methylation: Not Sensitive
- Heat Inactivation: No
- Restrictions
- For Research Use only
-
- Concentration
- 20000 U/mL
- Buffer
- 10 mM Tris-HCl ( pH 7.5), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, and 50 % (v/v) Glycerol
- Storage
- -20 °C
-
You are here:
