NcoI ClearCut
RD
20000 U/mL
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- Application
- Restriction Digest (RD)
- Characteristics
- ClearCut Enzymes are engineered to have high specificity (reduced star activity) and high activity (cuts in 5-15 minutes).
- Components
- NcoI ClearCut enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
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- Comment
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- Restriction enzyme digestion of doublestranded DNA
- Reaction: 1X Universal Restriction Enzyme Reaction Buffer. Incubate at 37°C
- Source: An E. coli strain that carries the modified NcoI gene from Nocardia carolina (ATCC 19070). The NcoI ClearCut enzyme recognizes and cuts the sequence C^CATGG.
- Reaction Buffer: Buffer 1: 50%, Buffer 2: 100%, Buffer 3: 10%, Universal Buffer: 100%
- Methylation Sensitivity
- dam methylation: Not Sensitive
- dcm methylation: Not Sensitive
- CpG Methylation: Not Sensitive
- Restrictions
- For Research Use only
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- Concentration
- 20000 U/mL
- Buffer
- 10 mM Tris-HCl ( pH 7.5), 200 mM NaCl, 1 mM DTT, 0.1 mM EDTA, and 50 % (v/v) Glycerol
- Storage
- -20 °C
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