RecA Protein
2 mg/mL
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- Characteristics
- RecA from E. coli is a DNA-binding protein that is involved in homologous recombination in an ATP-dependent process. RecA binds to single-stranded DNA forming a nucleoprotein complex and promotes the strand exchange of single-strand DNA fragments with homologous duplex DNA. RecA also plays a role in post-replicative DNA repair mechanisms and in DNA repair and UV-induced mutagenesis. RecA Protein is commonly used to study the molecular mechanisms involved in homologous recombination.
- Components
- Protein supplied with 10X Reaction Buffer.
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- Comment
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- displacement loop mutagenesis
- targeted DNA cleavage
- visualization of DNA with electron microscopy
- library screening with RecA coated probes
- Restrictions
- For Research Use only
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- Concentration
- 2 mg/mL
- Buffer
- 10 mM Tris-HCl ( pH 7.5), 0.1 mM EDTA, 1 mM DTT, and 50 % (v/v) Glycerol.
- Storage
- -20 °C
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