T4 DNA Ligase
Lig
1 U/μL
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- Application
- Ligation (Lig)
- Characteristics
- T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the proximal 3'- hydroxyl and 5'- phosphate ends of adjacent, nicked nucleotides of dsDNA, dsRNA or DNA-RNA hybrids. Known to proceed via an adenylated intermediate, this endergonic reaction can be utilized to join both cohesive-ended and blunt-ended termini.
- Components
- Enzyme supplied with 5X Reaction Buffer
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- Comment
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- Cloning of restriction fragments
- Attaching doublestranded oligonucleotide linkers or adaptors to DNA
- Sitedirected mutagenesis
- Nick repair of duplex DNA, RNA or DNARNA hybrids
- Selfcircularization of linear DNA
- Restrictions
- For Research Use only
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- Concentration
- 1 U/μL
- Storage
- -20 °C
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