T4 DNA Polymerase
DNA Amp
5 U/μL
-
- Application
- DNA Amplification (DNA Amp)
- Characteristics
- T4 DNA Polymerase catalyzes the 5'→3' synthesis of DNA from a single stranded, primed DNA template. This high fidelity enzyme also has potent 3'→5' exonuclease activity but lacks 5'→3' exonuclease function. T4 DNA Polymerase is the ideal choice for creating blunt ended DNA by removing 3'-overhangs or 5'-overhang filling, and is also useful for second strand DNA synthesis in site-specific mutagenesis.
- Components
- Enzyme supplied with 10X Reaction Buffer
- Unit Definition
- One unit is defined as the amount of T4 DNA Polymerase that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer.
-
-
- Comment
-
- Generates blunt end DNA by filling in 5'overhangs or/and removing 3'overhangs
- High fidelity due to strong 3'→5' exonuclease activity
- Synthesis of labeled DNA probes by the replacement reaction
- Sitespecific mutagenesis via primer extension from oligonucleotides
- Restrictions
- For Research Use only
-
- Concentration
- 5 U/μL
- Buffer
- 100 mM KPO4 ( pH 6.5), 1 mM DTT, and 50 % (v/v) Glycerol.
- Storage
- -20 °C
-
You are here:
