T4 RNA Ligase 1 (ssRNA Ligase)
Lig
10 U/μL
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- Application
- Ligation (Lig)
- Characteristics
- T4 RNA Ligase 1 (ssRNA Ligase) catalyzes the ligation of a single stranded 5' phosphoryl-termini donor to a 3' hydroxyl-termini nucleic acid acceptor through the formation of a 3'→5' phosphodiester bond, through hydrolysis of ATP to AMP and PPi . Substrates for T4 RNA Ligase I include single-stranded RNA and DNA as well as dinucleoside pyrophosphates.
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- Comment
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- ligation of a ssRNA and DNA.
- labeling of the 3' ends of RNA with 5'[32P]pCp.
- synthesis of singlestranded oligonucleotides
- Restrictions
- For Research Use only
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- Concentration
- 10 U/μL
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