Uracil DNA Glycosylase
5 U/μL
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- Characteristics
- E. coli Uracil DNA Glycosylase (UDG) catalyses the release of free uracil via hydrolysis of the N-glycosylic bond between the uracil and sugar from uracil containing single- or double- stranded DNA. This enzyme does not show activity on oligomers of 6 or less bases or on RNA.
- Components
- Enzyme supplied with 10X Reaction Buffer
- Unit Definition
- One unit is defined as the amount of Uracil DNA Glycosylase that catalyzes the release of 60 pmol of uracil per one minute from uracil-containing, double-stranded DNA at 37°C.
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- Comment
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- Prevents carryover contamination in PCR
- Glycosylase mediated polymorphism detection (GMPD)
- Sitedirected mutagenesis
- Restrictions
- For Research Use only
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- Concentration
- 5 U/μL
- Buffer
- 30 mM Tris-HCl ( pH 7.5), 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.15 % Tween-20 and 50 % (v/v) Glycerol.
- Storage
- -20 °C
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