Poly(A) Polymerase, Yeast
DNA Amp
1 U/μL
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- Application
- DNA Amplification (DNA Amp)
- Characteristics
- Poly(A) Polymerase, Yeast catalyses the template independent addition of adenosine residues onto the 3' ends of polyribonucleotides. The use of ATP as a substrate leads to poly(A) tailing whereas substitution of cordycepin-5'-triphosphate (3'-dATP) for ATP results in addition of a single dA residue to the 3'-termini of the RNA. Neither ADP nor dATP can be used as substrates for this enzyme. Poly(A) Polymerase from yeast has been shown to be more effective at oligonucleotide-labeling and poly(A) tailing of long RNA templates than Poly(A) Polymerase from E. coli.
- Components
- Poly(A) Polymerase, Yeast (1 U/µl) 100 µl, 5X Poly(A) Polymerase, Yeast Reaction Buffer 1 ml,25mM MnCl₂ 500 µl, ATP (10 mM) 150 µl
- Unit Definition
- One unit is defined as the amount of Poly(A) Polymerase, Yeast that catalyzes the incorporation of 1 nmol of AMP into RNA in 10 minutes at 37°C.
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- Comment
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- Labelling of RNA with ATP or cordycepin
- Poly(A) tailing of RNA for cloning or affinity purification
- Increasing translation of RNA transferred into eukaryotic cells
- Restrictions
- For Research Use only
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- Concentration
- 1 U/μL
- Buffer
- 20 mM Tris-HCl ( pH 8.0), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1 % Triton® X-100 and 50 % (v/v) Glycerol.
- Storage
- -20 °C
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