EasyTaq® DNA Polymerase for PAGE (with 2.5 mM dNTPs)
PCR
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- Application
- Polymerase Chain Reaction (PCR)
- Purpose
- EasyTaq® DNA Polymerase for PAGE is purified from E. coli expressing a cloned DNA polymerase from Thermus aquaticus.
- Brand
- EasyTaq®
- Specificity
- The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. EasyTaq® DNA Polymerase for PAGE has 5'-3' DNA polymerase activity and 5'-3' exonuclease activity. lt lacks 3'-5' exonuclease activity. This enzyme is supplied with unique buffer, and its PCR product is suitable for SDS-PAGE and agarose gel electrophoresis.
- Characteristics
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- Extension rate is about 1-2 kb/min.
- Unique buffer system compatible with PAGE.
- Template-independent "A" can be generated at the 3' end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
- Amplification of genomic DNA fragment up to 3 kb. - Components
- DNA Polymerase, 10X Taq Buffer, 2.5 mM dNTPs, 6X DNA Loading Buffer
- Unit Definition
- One unit of EasyTaq® DNA Polymerase for PAGE incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
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- Application Notes
- Short fragment PCR
- Comment
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EasyTaq® DNA Polymerase for PAGE has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity, >99% homogeneous measured by SDS-PAGE. Each batch of EasyTaq® DNA Polymerase for PAGE has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.
- Restrictions
- For Research Use only
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- Buffer
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Storage Buffer: 20 mM Tris-HCl ( pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50 % glycerol, stabilizers
10xEasyTaq® Buffer for PAGE (with Mg2+): 200 mM Tris-HCl ( pH 8.3), 200 mM KCl, 100 mM (NH4)2SO4, 20 mM MgSO4, others - Storage
- -20 °C
- Storage Comment
- at -20°C for two years
- Expiry Date
- 24 months
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