FlyCut™ SacI
RD
-
- Application
- Restriction Digest (RD)
- Purpose
- Super fast restriction enzyme
- Brand
- FlyCut™
- Specificity
-
5'...GAGCT^C...3'
3'...C^TCGAG...5' - Characteristics
- FlyCut® SacI is expressed and purified from E.coli that carries the recombinant SacI gene. The molecular weight is 40.8 kDa, with the recognition site at GAGCT^C. The reaction is conducted for 5-10 minutes at 37oC, and heat-inactivated at 80oC for 20 minutes.This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.
- Components
- Enzyme, 10X Buffer, 10X DNA Loading Buffer
- Unit Definition
- One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
-
-
- Application Notes
- Genomic DNA, plasmid DNA, PCR product
- Comment
-
Fast: 5 minutes DNA digestion.
Simple: universal buffer for all restriction enzymes. - Restrictions
- For Research Use only
-
- Buffer
-
Storage Buffer: 20 mM Tris-HCl pH 7.4, 250 mM NaCl, 0.1 mM EDTA, 1.5 mM DTT, 400 μg/mL BSA, 50 % Glycerol
10xFlyCut® Buffer: 500 mM Tris-Ac pH 7.9, 1 M KAc, 120 mM MgAc2, 1 mg/mL BSA - Storage
- -20 °C
- Storage Comment
- at -20°C for two years
- Expiry Date
- 24 months
-
You are here:
