TransScript® II Green One-Step qRT-PCR SuperMix
qPCR
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- Application
- Quantitative real-time PCR (qPCR)
- Purpose
- SuperMix with necessary reagents for cDNA synthesis and qPCR except total RNA/mRNA template and gene
- Brand
- TransScript®
- Specificity
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5 minutes cDNA synthesis
cDNA synthesis and qPCR are performed in a single tube using gene specific primers with total RNA or mRNA as templates.
Passive reference dyes are provided for different qPCR instruments. - Characteristics
- TransScript® II Green One-Step qRT-PCR SuperMix combines the first-strand cDNA synthesis and qPCR in the same tube to simplify reaction setup and reduce the possibility of contamination. Only gene specific primers can be used for this kit. TransScript® II Green One-Step qRT-PCR SuperMix contains all the necessary reagents for cDNA synthesis and qPCR except total RNA/mRNA template and gene specific primers.
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- Application Notes
- Multiple copy and low copy gene detection, GC-rich or complex secondary structure RNA template, Viral RNA and trace RNA detection
- Comment
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100 rxns x 20 μl Systems
- Restrictions
- For Research Use only
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- Storage
- -20 °C
- Storage Comment
- at -20°C for two years
- Expiry Date
- 24 months
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