TransScript® Reverse Transcriptase
cDNA
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- Application
- cDNA Synthesis (cDNA)
- Purpose
- TransScript® Reverse Transcriptase is a recombinant M-MLV reverse transcriptase with deficient RNase H activity.
- Brand
- TransScript®
- Specificity
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Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
cDNA up to 12 kb. - Components
- TransScript® Reverse Transcriptase, 5xTS RT Buffer, Anchored Oligo (dT) 18 Primer (0.5 μg/μL)
- Unit Definition
- One unit of TransScript® RT incorporates 1 nmol of deoxyribonucleotide into acid-precipitable material in 10 minutes at 37°C using Poly(A)/Oligo(dT) as template/primer.
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- Application Notes
- First-strand cDNA synthesis, Multiple copy and low copy gene detection
- Restrictions
- For Research Use only
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- Buffer
- 5xTS RT Buffer: 250 mM KCl, 15 mM MgCl2, 100 mM Tris-HCl pH 8.4
- Storage
- -20 °C
- Storage Comment
- at -20°C for two years
- Expiry Date
- 24 months
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