TransStart® FastPfu DNA Polymerase (with 2.5 mM dNTPs)
PCR
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- Application
- Polymerase Chain Reaction (PCR)
- Purpose
- TransStart® FastPfu DNA Polymerase is a fast, high fidelity and high processivity hot start DNA polymerase.
- Brand
- TransStart®
- Characteristics
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- Extension rate is about 2-4 kb/min.
- TransStart® FastPfu DNA Polymerase offers 54-fold fidelity as compared to EasyTaq® DNA Polymerase.
- PCR products can be directly cloned into pEASY®-Blunt vectors.
- Amplification of genomic DNA fragment up to 15 kb.
- Amplification of plasmid DNA fragment up to 20 kb. - Components
- DNA Polymerase, 10X Pfu Buffer, 2.5 mM dNTPs, PCR Stimulant, 6X DNA Loading Buffer, 50 mM MgSO4
- Unit Definition
- One unit of TransStart® FastPfu DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
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- Application Notes
- High fidelity PCR, High yield and fast PCR, Blunt end cloning, Site-directed mutagenesis, Complex templates
- Comment
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TransStart® FastPfu DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity, >99% homogeneous measured by SDS-PAGE. Each batch of TransStart® FastPfu DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.
- Restrictions
- For Research Use only
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- Buffer
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Storage Buffer: 50 mM Tris-HCl ( pH 8.2), 0.1 mM EDTA, 1 mM DTT, Stabilizers, 50 % glycerol
5xTransStart® FastPfu Buffer with 20 mM MgSO4: 100 mM Tris-SO4 ( pH 9.2), 50 mM (NH4)2SO4, 200 mM KCl, 10 mM MgSO4, 10 % Glycerol, others - Storage
- -20 °C
- Storage Comment
- at -20°C for two years
- Expiry Date
- 24 months
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