TransStart® KD Plus DNA Polymerase (with 2.5 mM dNTPs)
PCR
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- Application
- Polymerase Chain Reaction (PCR)
- Purpose
- TransStart® KD Plus DNA Polymerase is a genetically modified high fidelity DNA polymerase.
- Brand
- TransStart®
- Specificity
- This enzyme provides higher amplification capability than traditional Pfu DNA polymerase and fast amplification speed equal to Taq DNA polymerase (1 kb/min). Due to strong 3'-5' exonuclease activity, this enzyme offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase.
- Characteristics
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- PCR products can be directly cloned into pEASY®-Blunt vectors.
- Amplification of genomic DNA fragment up to 15 kb.
- Amplification of plasmid DNA fragment up to 20 kb. - Components
- DNA Polymerase, 5X Buffer, 2.5 mM dNTPs, 6X DNA Loading Buffer, 50 mM MgSO4
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- Application Notes
- Fast, high specificity amplification, High fidelity, high yield amplification
- Restrictions
- For Research Use only
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- Buffer
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Storage Buffer: 50 mM Tris-Cl ( pH 8.0),50 mM KCl,1 mM DTT,0.1 mM EDTA,Stabilizers,50 % glycerol.
5xTransStart® KD Plus Buffer with 20 mM MgSO4: 100 mM Tris-Cl ( pH 9.2),50 mM (NH4)2SO4,200 mM KCl,5 mM MgSO4,10 % glycerol, other. - Storage
- -20 °C
- Storage Comment
- at -20°C for two years
- Expiry Date
- 24 months
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