TransStart® TopTaqDNA Polymerase (with 2.5 mM dNTPs)
PCR
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- Application
- Polymerase Chain Reaction (PCR)
- Purpose
- TransStart® TopTaq DNA Polymerase is an engineered version of Taq DNA Polymerase combined with TransStart® technique.
- Brand
- TransStart®
- Specificity
- One binding protein binds to double-strand DNA template, preventing polymerase activity at room temperature. Other two binding proteins bind primers, preventing primer-dimer formation. Blocking proteins are released from primers and templates during the initial denaturation. This double blocking method has higher efficiency than antibody based, or chemically modified hot start PCR.
- Characteristics
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- Compared with TransStart® Taq DNA Polymerase, TransStart® TopTaq DNA Polymerase has higher amplification efficiency, specificity and sensitivity.
- TransStart® TopTaq DNA Polymerase offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
- The specificity is higher than antibody based or chemically modified hot start DNA polymerases.
- Template-independent "A" can be generated at the 3' end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
- Reduced nonspecific amplification and primer dimer formation.
- Different from Taq antibody, no risk of contamination from mammalian DNA.
- Different from chemical modification, long denaturing step is not needed.
- Amplification of genomic DNA fragment up to 15 kb. - Components
- DNA Polymerase, 10X Taq Buffer, 2.5 mM dNTPs, 10X GC Enhancer, 6X DNA Loading Buffer
- Unit Definition
- One unit of TransStart® TopTaq DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
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- Application Notes
- Complex templates, GC/AT-rich templates, Multiplex PCR, High yield PCR
- Comment
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TransStart® TopTaq DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity, >99% homogeneous measured by SDS-PAGE. Each batch of TransStart® TopTaq DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.
- Restrictions
- For Research Use only
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- Buffer
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Storage Buffer:20 mM Tris-HCl ( pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50 % glycerol, stabilizers.
10xTransStart® TopTaq Buffer with 20 mM MgSO4: 500 mM Tris-HCl ( pH 9.0), 200 mM (NH4)2 SO4, 20 mM MgSO4, others - Storage
- -20 °C
- Storage Comment
- at -20°C for two years
- Expiry Date
- 24 months
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