TransTaq® DNA Polymerase High Fidelity(HiFi)
PCR
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- Application
- Polymerase Chain Reaction (PCR)
- Purpose
- TransTaq® DNA Polymerase High Fidelity (TransTaq® HiFi DNA Polymerase) contains TransTaq®-T DNA Polymerase and a proofreading 3'-5' exonuclease.
- Brand
- TransTaq®
- Specificity
- TransTaq® HiFi DNA Polymerase provides higher specificity and higher amplification efficiency than TransTaq®-T DNA Polymerase. Two different buffers are provided in the kit. TransTaq® HiFi Buffer I is optimized for the amplification of genomic DNA and TransTaq® HiFi Buffer II is optimized for the amplification of λDNA, cDNA or plasmid DNA.
- Characteristics
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- TransTaq® HiFi DNA Polymerase offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
- Extension rate is about 1-2 kb/min.
- Template-independent "A" can be generated at the 3' end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
- Amplification of genomic DNA fragment up to 15 kb. - Components
- DNA Polymerase, 10X HiFi Buffer I, 10X HiFi Buffer II, 10X GC Enhancer, 6X DNA Loading Buffer
- Unit Definition
- One unit of TransTaq® HiFi DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.
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- Application Notes
- Complex templates, GC/AT rich templates, Long PCR, High yield PCR
- Comment
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TransTaq® HiFi DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity, >99% homogeneous measured by SDS-PAGE. Each batch of TransTaq® HiFi DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA
- Restrictions
- For Research Use only
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- Buffer
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Storage Buffer: 20 mM Tris-HCl ( pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50 % glycerol, stabilizers
10xTransTaq® HiFi Buffer:200 mM Tris-HCl ( pH 9.0), 100 mM KCl, 100 mM (NH4)2SO4, 20 mM MgSO4, 10 % Glycerol, others - Storage
- -20 °C
- Storage Comment
- at -20°C for two years
- Expiry Date
- 24 months
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