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Micrococcal Nuclease S7

Rockland mb-116-0015
Rockland
Catalog No. ABIN5706732
Supplier Product No.: mb-116-0015
  • Species
    Staphylococcus aureus
    Supplier Product No.
    mb-116-0015
    Supplier
    Rockland
    Purpose
    Micrococcal Nuclease S7
    Characteristics
    Micrococcal nuclease catalyzes cleavage of both DNA and RNA to yield 3'-nucleotides. It exhibits exo- and endo-5'-phosphodiesterase activities. The enzyme catalyzes preferential endohydrolysis of the RNA and DNA at sites rich in adenylate or uridylate and deoxyadenylate or thymidylate. The enzyme has a molecular weight of 16,807 daltons and is calcium dependent. The pH optimum is 9.2 but varies depending upon the concentration of ionized calcium present.
    Purification
    Purified by chromatography and assayed by SDS-PAGE.
    Unit Definition
    One Unit causes a change in optical density of 1.0 at 260 nm at 37°C, pH 8.0, using DNA as the substrate.
    Biological Activity Comment
    6,000 units per mg protein
  • Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Restore with 1 mL deionized water (or equivalent).
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C or at -20° C or colder prior to opening product.
    Expiry Date
    6 months
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