cDNA Synthesis

Complementary DNA (cDNA) is synthesized in a reverse transcription reaction by the enzyme reverse transcriptase from a single-stranded mRNA template. cDNA molecules are commonly used to clone and express eukaryotic genes in prokaryotes since the original genes cannot be readily cloned due to the presence of introns. Our reverse transcriptase and cDNA synthesis premixes provide you with the necessary tools for cDNA first-strand synthesis and PCR amplification specifically from mRNA or total RNA extracts.