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CHFR (Checkpoint with Forkhead and Ring Finger Domains, CHFR)

Short Description: E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating ubc13-mms2 (ube2n-ube2v2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress (By similarity).
More information related to gene CHFR.

Products related to CHFR Gene:

80 results :

Human CHFR cDNA Clone in Bacterial Expression Vector (His-GST)
cDNA Clon Bacterial Expression Vector pPB-His-GST Kanamycin T7 Promoter His-GST Transient
Type:
cDNA
Application:
Cloning
Gene:
Checkpoint with Forkhead and Ring Finger Domains (CHFR)
Species:
Human
Vector type:
Bacterial Expression Vector
Vector backbone:
pPB-His-GST
Resistance Gene:
Kanamycin
Promoter:
T7 Promoter
Fusion Tag:
His-GST
Expression:
Transient
Catalog No. ABIN4828968
Datasheet
Catalog No. ABIN4828968
Type:
cDNA
Application:
Cloning
Gene:
Checkpoint with Forkhead and Ring Finger Domains (CHFR)
Species:
Human
Synonyms:
CHFR, chfr, Chfr, chfr.L
Insert length:
1959 bp
Vector type:
Bacterial Expression Vector
Vector backbone:
pPB-His-GST
Resistance Gene:
Kanamycin
Promoter:
T7 Promoter
Fusion Tag:
His-GST
Expression:
Transient
Sequencing Primer:
  • GST Forward primer: 5'-CACGTTTGGTGGTGGCGAC3'
  • T7 terminator primer: 5'-GCTAGTTATTGCTCAGCGG-3'
Format:
Liquid
Buffer:
10 mM Tris-HCI, 1 mM EDTA, pH 8.0
Storage:
-20 °C
Human CHFR cDNA Clone in Bacterial Expression Vector (His-MBP)
cDNA Clon Bacterial Expression Vector pPB-His-MBP Kanamycin T7 Promoter His-MBP Transient
Type:
cDNA
Application:
Cloning
Gene:
Checkpoint with Forkhead and Ring Finger Domains (CHFR)
Species:
Human
Vector type:
Bacterial Expression Vector
Vector backbone:
pPB-His-MBP
Resistance Gene:
Kanamycin
Promoter:
T7 Promoter
Fusion Tag:
His-MBP
Expression:
Transient
Catalog No. ABIN4699839
Datasheet
Catalog No. ABIN4699839
Type:
cDNA
Application:
Cloning
Gene:
Checkpoint with Forkhead and Ring Finger Domains (CHFR)
Species:
Human
Synonyms:
CHFR, chfr, Chfr, chfr.L
Insert length:
1959 bp
Vector type:
Bacterial Expression Vector
Vector backbone:
pPB-His-MBP
Resistance Gene:
Kanamycin
Promoter:
T7 Promoter
Fusion Tag:
His-MBP
Expression:
Transient
Sequencing Primer:
  • MBP Forward primer: 5'-CGCAGATGTCCGCTTTCTGG-3'
  • T7 terminator primer: 5'-GCTAGTTATTGCTCAGCGG-3'
Format:
Liquid
Buffer:
10 mM Tris-HCI, 1 mM EDTA, pH 8.0
Storage:
-20 °C
Human CHFR cDNA Clone in Mammalian Expression Vector (HA tag)
cDNA PExp Mammalian Expression Vector pPM-C-HA Kanamycin CMV Promoter HA tag Stable
Type:
cDNA
Application:
Protein Expression
Gene:
Checkpoint with Forkhead and Ring Finger Domains (CHFR)
Species:
Human
Vector type:
Mammalian Expression Vector
Vector backbone:
pPM-C-HA
Resistance Gene:
Kanamycin
Selection Marker:
Neomycin
Promoter:
CMV Promoter
Fusion Tag:
HA tag
Expression:
Stable, Transient
Catalog No. ABIN4487443
Datasheet
Catalog No. ABIN4487443
Type:
cDNA
Application:
Protein Expression
Gene:
Checkpoint with Forkhead and Ring Finger Domains (CHFR)
Species:
Human
Synonyms:
CHFR, chfr, Chfr, chfr.L
Insert length:
1959 bp
Vector type:
Mammalian Expression Vector
Vector backbone:
pPM-C-HA
Resistance Gene:
Kanamycin
Selection Marker:
Neomycin
Promoter:
CMV Promoter
Fusion Tag:
HA tag
Expression:
Stable, Transient
Sequencing Primer:
  • CMV sequencing primer: 5` CGC AAA TGG GCG GTA GGC GTG 3`
Format:
Liquid
Buffer:
10 mM Tris-HCI, 1 mM EDTA, pH 8.0
Storage:
-20 °C
Human CHFR ORF Clone in Cloning Vector
ORF Clon Cloning Vector pORF Spectinomycin unconjugated Transient
Type:
ORF
Application:
Cloning
Gene:
Checkpoint with Forkhead and Ring Finger Domains (CHFR)
Species:
Human
Vector type:
Cloning Vector
Vector backbone:
pORF
Resistance Gene:
Spectinomycin
Expression:
Transient
Catalog No. ABIN5764364
Datasheet
Catalog No. ABIN5764364
Type:
ORF
Application:
Cloning
Gene:
Checkpoint with Forkhead and Ring Finger Domains (CHFR)
Species:
Human
Synonyms:
CHFR, chfr, Chfr, chfr.L
Insert length:
1959 bp
Vector type:
Cloning Vector
Vector backbone:
pORF
Resistance Gene:
Spectinomycin
Expression:
Transient
Sequencing Primer:
  • M13 FP: 5'-CCCAGTCACGACGTTGTAAAACG-3'
    M13 RP: 5'-CAGGAAACAGCTATGAC-3'
Format:
Liquid
Buffer:
10  mM Tris-HCI, 1  mM EDTA,  pH 8.0
Storage:
-20 °C
Rat CHFR CRISPR gRNA in Lenti Particles
gRNA PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-PGK-Neo Ampicillin U6 Promoter unconjugated Stable
Type:
gRNA
Application:
Protein Expression, Genome Editing with Engineered Nucleases
Gene:
Checkpoint with Forkhead and Ring Finger Domains (CHFR)
NCBI Accession:
NM_001009258
Species:
Rat
Vector type:
Lentiviral Vector
Vector backbone:
pLenti-U6-sgRNA-PGK-Neo
Resistance Gene:
Ampicillin
Selection Marker:
Neomycin
Promoter:
U6 Promoter, PGK Promoter
Expression:
Stable, Transient
Catalog No. ABIN5165208
Datasheet
Catalog No. ABIN5165208
Type:
gRNA
Application:
Protein Expression, Genome Editing with Engineered Nucleases
Gene:
Checkpoint with Forkhead and Ring Finger Domains (CHFR)
NCBI Accession:
NM_001009258
Species:
Rat
Synonyms:
CHFR, chfr, Chfr, chfr.L
Vector type:
Lentiviral Vector
Vector backbone:
pLenti-U6-sgRNA-PGK-Neo
Resistance Gene:
Ampicillin
Selection Marker:
Neomycin
Promoter:
U6 Promoter, PGK Promoter
Expression:
Stable, Transient
Components:
Lentiviral particles with an individual gRNA (300 μL) for a specific sequence of Chfr
Format:
Viral Particles
Storage:
-80 °C
Mouse CHFR CRISPR gRNA in Lenti Particles
gRNA PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-PGK-Neo Ampicillin U6 Promoter unconjugated Stable
Type:
gRNA
Application:
Protein Expression, Genome Editing with Engineered Nucleases
Gene:
Checkpoint with Forkhead and Ring Finger Domains (CHFR)
NCBI Accession:
NM_172717
Species:
Mouse
Vector type:
Lentiviral Vector
Vector backbone:
pLenti-U6-sgRNA-PGK-Neo
Resistance Gene:
Ampicillin
Selection Marker:
Neomycin
Promoter:
U6 Promoter, PGK Promoter
Expression:
Stable, Transient
Catalog No. ABIN5165206
Datasheet
Catalog No. ABIN5165206
Type:
gRNA
Application:
Protein Expression, Genome Editing with Engineered Nucleases
Gene:
Checkpoint with Forkhead and Ring Finger Domains (CHFR)
NCBI Accession:
NM_172717
Species:
Mouse
Synonyms:
CHFR, chfr, Chfr, chfr.L
Vector type:
Lentiviral Vector
Vector backbone:
pLenti-U6-sgRNA-PGK-Neo
Resistance Gene:
Ampicillin
Selection Marker:
Neomycin
Promoter:
U6 Promoter, PGK Promoter
Expression:
Stable, Transient
Components:
Lentiviral particles with an individual gRNA (300 μL) for a specific sequence of Chfr
Format:
Viral Particles
Storage:
-80 °C
Human CHFR CRISPR gRNA in Lenti Particles
gRNA PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-PGK-Neo Ampicillin U6 Promoter unconjugated Stable
Type:
gRNA
Application:
Protein Expression, Genome Editing with Engineered Nucleases
Gene:
Checkpoint with Forkhead and Ring Finger Domains (CHFR)
NCBI Accession:
NM_018223
Species:
Human
Vector type:
Lentiviral Vector
Vector backbone:
pLenti-U6-sgRNA-PGK-Neo
Resistance Gene:
Ampicillin
Selection Marker:
Neomycin
Promoter:
U6 Promoter, PGK Promoter
Expression:
Stable, Transient
Catalog No. ABIN5165204
Datasheet
Catalog No. ABIN5165204
Type:
gRNA
Application:
Protein Expression, Genome Editing with Engineered Nucleases
Gene:
Checkpoint with Forkhead and Ring Finger Domains (CHFR)
NCBI Accession:
NM_018223
Species:
Human
Synonyms:
CHFR, chfr, Chfr, chfr.L
Vector type:
Lentiviral Vector
Vector backbone:
pLenti-U6-sgRNA-PGK-Neo
Resistance Gene:
Ampicillin
Selection Marker:
Neomycin
Promoter:
U6 Promoter, PGK Promoter
Expression:
Stable, Transient
Components:
Lentiviral particles with an individual gRNA (300 μL) for a specific sequence of CHFR
Format:
Viral Particles
Storage:
-80 °C
Human CHFR cDNA Clone in Bacterial Expression Vector (His tag)
cDNA Clon Bacterial Expression Vector pPB-N-His Kanamycin T7 Promoter His tag Transient
Type:
cDNA
Application:
Cloning
Gene:
Checkpoint with Forkhead and Ring Finger Domains (CHFR)
Species:
Human
Vector type:
Bacterial Expression Vector
Vector backbone:
pPB-N-His
Resistance Gene:
Kanamycin
Promoter:
T7 Promoter
Fusion Tag:
His tag
Expression:
Transient
Catalog No. ABIN4630913
Datasheet
Catalog No. ABIN4630913
Type:
cDNA
Application:
Cloning
Gene:
Checkpoint with Forkhead and Ring Finger Domains (CHFR)
Species:
Human
Synonyms:
CHFR, chfr, Chfr, chfr.L
Insert length:
1959 bp
Vector type:
Bacterial Expression Vector
Vector backbone:
pPB-N-His
Resistance Gene:
Kanamycin
Promoter:
T7 Promoter
Fusion Tag:
His tag
Expression:
Transient
Sequencing Primer:
  • T7 promoter primer: 5'-TAATACGACTCACTATAGGG-3'
  • T7 terminator primer: 5'-GCTAGTTATTGCTCAGCGG-3'
Format:
Liquid
Buffer:
10 mM Tris-HCI, 1 mM EDTA, pH 8.0
Storage:
-20 °C
Human CHFR cDNA Clone in Mammalian Expression Vector (His tag)
cDNA PExp Mammalian Expression Vector pPM-C-His Kanamycin CMV Promoter His tag Stable
Type:
cDNA
Application:
Protein Expression
Gene:
Checkpoint with Forkhead and Ring Finger Domains (CHFR)
Species:
Human
Vector type:
Mammalian Expression Vector
Vector backbone:
pPM-C-His
Resistance Gene:
Kanamycin
Selection Marker:
Neomycin
Promoter:
CMV Promoter
Fusion Tag:
His tag
Expression:
Stable, Transient
Catalog No. ABIN4422356
Datasheet
Catalog No. ABIN4422356
Type:
cDNA
Application:
Protein Expression
Gene:
Checkpoint with Forkhead and Ring Finger Domains (CHFR)
Species:
Human
Synonyms:
CHFR, chfr, Chfr, chfr.L
Insert length:
1959 bp
Vector type:
Mammalian Expression Vector
Vector backbone:
pPM-C-His
Resistance Gene:
Kanamycin
Selection Marker:
Neomycin
Promoter:
CMV Promoter
Fusion Tag:
His tag
Expression:
Stable, Transient
Sequencing Primer:
  • CMV sequencing primer: 5` CGC AAA TGG GCG GTA GGC GTG 3`
Format:
Liquid
Buffer:
10 mM Tris-HCI, 1 mM EDTA, pH 8.0
Storage:
-20 °C
Mouse CHFR ORF Clone in Cloning Vector
ORF Clon Cloning Vector pORF Spectinomycin unconjugated Transient
Type:
ORF
Application:
Cloning
Gene:
Checkpoint with Forkhead and Ring Finger Domains (CHFR)
NCBI Accession:
NM_172717
Species:
Mouse
Vector type:
Cloning Vector
Vector backbone:
pORF
Resistance Gene:
Spectinomycin
Expression:
Transient
Catalog No. ABIN5764365
Datasheet
Catalog No. ABIN5764365
Type:
ORF
Application:
Cloning
Gene:
Checkpoint with Forkhead and Ring Finger Domains (CHFR)
NCBI Accession:
NM_172717
Species:
Mouse
Synonyms:
CHFR, chfr, Chfr, chfr.L
Insert length:
1992 bp
Vector type:
Cloning Vector
Vector backbone:
pORF
Resistance Gene:
Spectinomycin
Expression:
Transient
Sequencing Primer:
  • M13 FP: 5'-CCCAGTCACGACGTTGTAAAACG-3'
    M13 RP: 5'-CAGGAAACAGCTATGAC-3'
Format:
Liquid
Buffer:
10  mM Tris-HCI, 1  mM EDTA,  pH 8.0
Storage:
-20 °C
  • Type
    • Plasmid
    • Primer
  • Supplier
    • OriGene
    • antibodies-online
    • GenScript
  • Reactivity
    • Human
    • Mouse
    • Rat
  • Application
    • Protein Expression (PExp)
    • RNA Interference (RNAi)
    • Genome Editing with Engineered Nucleases (GEEN)
    • Cloning (Clon)
  • Conjugate
    • Un-conjugated
    • GFP tag
    • Myc-DYKDDDDK Tag
    • His tag
    • DYKDDDDK Tag
    • HA tag
    • His-GST
    • His-MBP
    • RFP tag
  • Vector Backbone
    • pCMV6-Entry
    • pLenti-U6-sgRNA-PGK-Neo
    • pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro
    • pCMV6-AC-GFP
    • pcDNA3.1+C-(K)-DYK
    • pLenti-C-Myc-DDK
    • pLenti-C-mGFP
    • pGFP-C-shLenti
    • pGFP-V-RS
    • pORF
    • pPB-His-GST
    • pPB-His-MBP
    • pPB-N-His
    • pPM-C-HA
    • pPM-C-His
    • pRS
    • pCas-Guide
    • pRFP-C-RS
    • pCMV6-XL5
  • Vector
    • Mammalian Expression Vector
    • Lentiviral Vector
    • Bacterial Expression Vector
    • Retroviral Vector
    • Cloning Vector
  • Insert
    • ORF
    • cDNA
    • shRNA
    • gRNA + Cas9
    • gRNA
  • Type of Oligonucleotide
    • siRNA Oligo
  • Bacterial Resistance
    • Kanamycin
    • Ampicillin
    • Chloramphenicol
    • Spectinomycin
  • Expression Type
    • Transient
    • Stable
  • Selectable Marker
    • Neomycin
    • Puromycin
  • Promoter
    • CMV Promoter
    • U6 Promoter
    • Enhanced CMV Promoter
    • T7 Promoter
    • PGK Promoter
    • SFFV Promoter
  • Format
    • Lyophilized
    • Liquid
    • Viral Particles

Synonyms and alternative names related to CHFR

  • checkpoint with forkhead and ring finger domains (CHFR)
  • checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase (chfr)
  • checkpoint with forkhead and ring finger domains (Chfr)
  • checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase L homeolog (chfr.L)
  • 5730484M20Rik
  • C230082M18
  • CHFR
  • fc43f10
  • RNF116
  • rnf116
  • RNF196
  • rnf196
  • si:dkey-69h6.7
  • wu:fc43f10

Gene-IDs for different species

Protein level used designations for CHFR

  • E3 ubiquitin-protein ligase CHFR
  • RING finger protein 196
  • checkpoint with forkhead and RING finger domains protein
  • checkpoint with forkhead and ring finger domains
  • Checkpoint with forkhead and RING finger domains protein
  • e3 ubiquitin-protein ligase CHFR-like
Other products related to CHFR such as antibodies, ELISA kits and high-purity proteins are available on our partner website antibodies-online.com
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