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DBT (Dihydrolipoamide Branched Chain Transacylase E2, DBT)

Short Description: The branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine. The BCKD complex is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. This gene encodes the transacylase (E2) subunit. Mutations in this gene result in maple syrup urine disease, type 2. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008].
More information related to gene DBT.

Products related to DBT Gene:

56 results :

Human DBT ORF Clone in Cloning Vector
ORF Clon Cloning Vector pORF Spectinomycin unconjugated Transient
Type:
ORF
Application:
Cloning
Gene:
Dihydrolipoamide Branched Chain Transacylase E2 (DBT)
NCBI Accession:
NM_001918
Species:
Human
Vector type:
Cloning Vector
Vector backbone:
pORF
Resistance Gene:
Spectinomycin
Expression:
Transient
Catalog No. ABIN5739776
Datasheet
Catalog No. ABIN5739776
Type:
ORF
Application:
Cloning
Gene:
Dihydrolipoamide Branched Chain Transacylase E2 (DBT)
NCBI Accession:
NM_001918
Species:
Human
Synonyms:
DBT, dbt, CpipJ_CPIJ006326, BDBG_05874, NAEGRDRAFT_78509, VDBG_04820, PGTG_17722, Dbt, dbt.L
Insert length:
1449 bp
Vector type:
Cloning Vector
Vector backbone:
pORF
Resistance Gene:
Spectinomycin
Expression:
Transient
Sequencing Primer:
  • M13 FP: 5'-CCCAGTCACGACGTTGTAAAACG-3'
    M13 RP: 5'-CAGGAAACAGCTATGAC-3'
Format:
Liquid
Buffer:
10  mM Tris-HCI, 1  mM EDTA,  pH 8.0
Storage:
-20 °C
Rat DBT CRISPR gRNA + Cas9 in Lenti Particles
gRNA + Cas9 PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro Ampicillin U6 Promoter unconjugated Stable
Type:
gRNA + Cas9
Application:
Protein Expression, Genome Editing with Engineered Nucleases
Gene:
Dihydrolipoamide Branched Chain Transacylase E2 (DBT)
NCBI Accession:
NM_053312
Species:
Rat
Vector type:
Lentiviral Vector
Resistance Gene:
Ampicillin
Selection Marker:
Puromycin
Promoter:
U6 Promoter, SFFV Promoter
Expression:
Stable, Transient
Catalog No. ABIN5232802
Datasheet
Catalog No. ABIN5232802
Type:
gRNA + Cas9
Application:
Protein Expression, Genome Editing with Engineered Nucleases
Gene:
Dihydrolipoamide Branched Chain Transacylase E2 (DBT)
NCBI Accession:
NM_053312
Species:
Rat
Synonyms:
DBT, dbt, CpipJ_CPIJ006326, BDBG_05874, NAEGRDRAFT_78509, VDBG_04820, PGTG_17722, Dbt, dbt.L
Vector type:
Lentiviral Vector
Vector backbone:
pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro
Resistance Gene:
Ampicillin
Selection Marker:
Puromycin
Promoter:
U6 Promoter, SFFV Promoter
Expression:
Stable, Transient
Sequencing Primer:
  • U6 Forward Primer: 5'--TACGTCCAAGGTCGGGCAGGAAGA--3'
Components:
Lentiviral particles with an individual gRNA (300 μL) for a specific sequence of Dbt
Format:
Viral Particles
Storage:
-80 °C
Mouse DBT CRISPR gRNA + Cas9 in Lenti Particles
gRNA + Cas9 PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro Ampicillin U6 Promoter unconjugated Stable
Type:
gRNA + Cas9
Application:
Protein Expression, Genome Editing with Engineered Nucleases
Gene:
Dihydrolipoamide Branched Chain Transacylase E2 (DBT)
NCBI Accession:
NM_010022
Species:
Mouse
Vector type:
Lentiviral Vector
Resistance Gene:
Ampicillin
Selection Marker:
Puromycin
Promoter:
U6 Promoter, SFFV Promoter
Expression:
Stable, Transient
Catalog No. ABIN5232800
Datasheet
Catalog No. ABIN5232800
Type:
gRNA + Cas9
Application:
Protein Expression, Genome Editing with Engineered Nucleases
Gene:
Dihydrolipoamide Branched Chain Transacylase E2 (DBT)
NCBI Accession:
NM_010022
Species:
Mouse
Synonyms:
DBT, dbt, CpipJ_CPIJ006326, BDBG_05874, NAEGRDRAFT_78509, VDBG_04820, PGTG_17722, Dbt, dbt.L
Vector type:
Lentiviral Vector
Vector backbone:
pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro
Resistance Gene:
Ampicillin
Selection Marker:
Puromycin
Promoter:
U6 Promoter, SFFV Promoter
Expression:
Stable, Transient
Sequencing Primer:
  • U6 Forward Primer: 5'--TACGTCCAAGGTCGGGCAGGAAGA--3'
Components:
Lentiviral particles with an individual gRNA (300 μL) for a specific sequence of Dbt
Format:
Viral Particles
Storage:
-80 °C
Human DBT CRISPR gRNA + Cas9 in Lenti Particles
gRNA + Cas9 PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro Ampicillin U6 Promoter unconjugated Stable
Type:
gRNA + Cas9
Application:
Protein Expression, Genome Editing with Engineered Nucleases
Gene:
Dihydrolipoamide Branched Chain Transacylase E2 (DBT)
NCBI Accession:
NM_001918
Species:
Human
Vector type:
Lentiviral Vector
Resistance Gene:
Ampicillin
Selection Marker:
Puromycin
Promoter:
U6 Promoter, SFFV Promoter
Expression:
Stable, Transient
Catalog No. ABIN5232798
Datasheet
Catalog No. ABIN5232798
Type:
gRNA + Cas9
Application:
Protein Expression, Genome Editing with Engineered Nucleases
Gene:
Dihydrolipoamide Branched Chain Transacylase E2 (DBT)
NCBI Accession:
NM_001918
Species:
Human
Synonyms:
DBT, dbt, CpipJ_CPIJ006326, BDBG_05874, NAEGRDRAFT_78509, VDBG_04820, PGTG_17722, Dbt, dbt.L
Vector type:
Lentiviral Vector
Vector backbone:
pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro
Resistance Gene:
Ampicillin
Selection Marker:
Puromycin
Promoter:
U6 Promoter, SFFV Promoter
Expression:
Stable, Transient
Sequencing Primer:
  • U6 Forward Primer: 5'--TACGTCCAAGGTCGGGCAGGAAGA--3'
Components:
Lentiviral particles with an individual gRNA (300 μL) for a specific sequence of DBT
Format:
Viral Particles
Storage:
-80 °C
Mouse DBT CRISPR gRNA in Lenti Particles
gRNA PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-PGK-Neo Ampicillin U6 Promoter unconjugated Stable
Type:
gRNA
Application:
Protein Expression, Genome Editing with Engineered Nucleases
Gene:
Dihydrolipoamide Branched Chain Transacylase E2 (DBT)
NCBI Accession:
NM_010022
Species:
Mouse
Vector type:
Lentiviral Vector
Vector backbone:
pLenti-U6-sgRNA-PGK-Neo
Resistance Gene:
Ampicillin
Selection Marker:
Neomycin
Promoter:
U6 Promoter, PGK Promoter
Expression:
Stable, Transient
Catalog No. ABIN5117442
Datasheet
Catalog No. ABIN5117442
Type:
gRNA
Application:
Protein Expression, Genome Editing with Engineered Nucleases
Gene:
Dihydrolipoamide Branched Chain Transacylase E2 (DBT)
NCBI Accession:
NM_010022
Species:
Mouse
Synonyms:
DBT, dbt, CpipJ_CPIJ006326, BDBG_05874, NAEGRDRAFT_78509, VDBG_04820, PGTG_17722, Dbt, dbt.L
Vector type:
Lentiviral Vector
Vector backbone:
pLenti-U6-sgRNA-PGK-Neo
Resistance Gene:
Ampicillin
Selection Marker:
Neomycin
Promoter:
U6 Promoter, PGK Promoter
Expression:
Stable, Transient
Components:
Lentiviral particles with an individual gRNA (300 μL) for a specific sequence of Dbt
Format:
Viral Particles
Storage:
-80 °C
Human DBT CRISPR gRNA in Lenti Particles
gRNA PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-PGK-Neo Ampicillin U6 Promoter unconjugated Stable
Type:
gRNA
Application:
Protein Expression, Genome Editing with Engineered Nucleases
Gene:
Dihydrolipoamide Branched Chain Transacylase E2 (DBT)
NCBI Accession:
NM_001918
Species:
Human
Vector type:
Lentiviral Vector
Vector backbone:
pLenti-U6-sgRNA-PGK-Neo
Resistance Gene:
Ampicillin
Selection Marker:
Neomycin
Promoter:
U6 Promoter, PGK Promoter
Expression:
Stable, Transient
Catalog No. ABIN5117440
Datasheet
Catalog No. ABIN5117440
Type:
gRNA
Application:
Protein Expression, Genome Editing with Engineered Nucleases
Gene:
Dihydrolipoamide Branched Chain Transacylase E2 (DBT)
NCBI Accession:
NM_001918
Species:
Human
Synonyms:
DBT, dbt, CpipJ_CPIJ006326, BDBG_05874, NAEGRDRAFT_78509, VDBG_04820, PGTG_17722, Dbt, dbt.L
Vector type:
Lentiviral Vector
Vector backbone:
pLenti-U6-sgRNA-PGK-Neo
Resistance Gene:
Ampicillin
Selection Marker:
Neomycin
Promoter:
U6 Promoter, PGK Promoter
Expression:
Stable, Transient
Components:
Lentiviral particles with an individual gRNA (300 μL) for a specific sequence of DBT
Format:
Viral Particles
Storage:
-80 °C
Rat DBT CRISPR gRNA in Lenti Particles
gRNA PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-PGK-Neo Ampicillin U6 Promoter unconjugated Stable
Type:
gRNA
Application:
Protein Expression, Genome Editing with Engineered Nucleases
Gene:
Dihydrolipoamide Branched Chain Transacylase E2 (DBT)
NCBI Accession:
NM_053312
Species:
Rat
Vector type:
Lentiviral Vector
Vector backbone:
pLenti-U6-sgRNA-PGK-Neo
Resistance Gene:
Ampicillin
Selection Marker:
Neomycin
Promoter:
U6 Promoter, PGK Promoter
Expression:
Stable, Transient
Catalog No. ABIN5117444
Datasheet
Catalog No. ABIN5117444
Type:
gRNA
Application:
Protein Expression, Genome Editing with Engineered Nucleases
Gene:
Dihydrolipoamide Branched Chain Transacylase E2 (DBT)
NCBI Accession:
NM_053312
Species:
Rat
Synonyms:
DBT, dbt, CpipJ_CPIJ006326, BDBG_05874, NAEGRDRAFT_78509, VDBG_04820, PGTG_17722, Dbt, dbt.L
Vector type:
Lentiviral Vector
Vector backbone:
pLenti-U6-sgRNA-PGK-Neo
Resistance Gene:
Ampicillin
Selection Marker:
Neomycin
Promoter:
U6 Promoter, PGK Promoter
Expression:
Stable, Transient
Components:
Lentiviral particles with an individual gRNA (300 μL) for a specific sequence of Dbt
Format:
Viral Particles
Storage:
-80 °C
Mouse DBT ORF Clone in Cloning Vector
ORF Clon Cloning Vector pORF Spectinomycin unconjugated Transient
Type:
ORF
Application:
Cloning
Gene:
Dihydrolipoamide Branched Chain Transacylase E2 (DBT)
NCBI Accession:
NM_010022
Species:
Mouse
Vector type:
Cloning Vector
Vector backbone:
pORF
Resistance Gene:
Spectinomycin
Expression:
Transient
Catalog No. ABIN5739777
Datasheet
Catalog No. ABIN5739777
Type:
ORF
Application:
Cloning
Gene:
Dihydrolipoamide Branched Chain Transacylase E2 (DBT)
NCBI Accession:
NM_010022
Species:
Mouse
Synonyms:
DBT, dbt, CpipJ_CPIJ006326, BDBG_05874, NAEGRDRAFT_78509, VDBG_04820, PGTG_17722, Dbt, dbt.L
Insert length:
1449 bp
Vector type:
Cloning Vector
Vector backbone:
pORF
Resistance Gene:
Spectinomycin
Expression:
Transient
Sequencing Primer:
  • M13 FP: 5'-CCCAGTCACGACGTTGTAAAACG-3'
    M13 RP: 5'-CAGGAAACAGCTATGAC-3'
Format:
Liquid
Buffer:
10  mM Tris-HCI, 1  mM EDTA,  pH 8.0
Storage:
-20 °C
Rat DBT ORF Clone in Cloning Vector
ORF Clon Cloning Vector pORF Spectinomycin unconjugated Transient
Type:
ORF
Application:
Cloning
Gene:
Dihydrolipoamide Branched Chain Transacylase E2 (DBT)
NCBI Accession:
NM_053312
Species:
Rat
Vector type:
Cloning Vector
Vector backbone:
pORF
Resistance Gene:
Spectinomycin
Expression:
Transient
Catalog No. ABIN5739778
Datasheet
Catalog No. ABIN5739778
Type:
ORF
Application:
Cloning
Gene:
Dihydrolipoamide Branched Chain Transacylase E2 (DBT)
NCBI Accession:
NM_053312
Species:
Rat
Synonyms:
DBT, dbt, CpipJ_CPIJ006326, BDBG_05874, NAEGRDRAFT_78509, VDBG_04820, PGTG_17722, Dbt, dbt.L
Insert length:
1449 bp
Vector type:
Cloning Vector
Vector backbone:
pORF
Resistance Gene:
Spectinomycin
Expression:
Transient
Sequencing Primer:
  • M13 FP: 5'-CCCAGTCACGACGTTGTAAAACG-3'
    M13 RP: 5'-CAGGAAACAGCTATGAC-3'
Format:
Liquid
Buffer:
10  mM Tris-HCI, 1  mM EDTA,  pH 8.0
Storage:
-20 °C
Human DBT cDNA Clone in Mammalian Expression Vector (HA tag)
cDNA PExp Mammalian Expression Vector pPM-C-HA Kanamycin CMV Promoter HA tag Stable
Type:
cDNA
Application:
Protein Expression
Gene:
Dihydrolipoamide Branched Chain Transacylase E2 (DBT)
NCBI Accession:
NM_001918
Species:
Human
Vector type:
Mammalian Expression Vector
Vector backbone:
pPM-C-HA
Resistance Gene:
Kanamycin
Selection Marker:
Neomycin
Promoter:
CMV Promoter
Fusion Tag:
HA tag
Expression:
Stable, Transient
Catalog No. ABIN4487679
Datasheet
Catalog No. ABIN4487679
Type:
cDNA
Application:
Protein Expression
Gene:
Dihydrolipoamide Branched Chain Transacylase E2 (DBT)
NCBI Accession:
NM_001918
Species:
Human
Synonyms:
DBT, dbt, CpipJ_CPIJ006326, BDBG_05874, NAEGRDRAFT_78509, VDBG_04820, PGTG_17722, Dbt, dbt.L
Insert length:
1449 bp
Vector type:
Mammalian Expression Vector
Vector backbone:
pPM-C-HA
Resistance Gene:
Kanamycin
Selection Marker:
Neomycin
Promoter:
CMV Promoter
Fusion Tag:
HA tag
Expression:
Stable, Transient
Sequencing Primer:
  • CMV sequencing primer: 5` CGC AAA TGG GCG GTA GGC GTG 3`
Format:
Liquid
Buffer:
10 mM Tris-HCI, 1 mM EDTA, pH 8.0
Storage:
-20 °C
  • Type
    • Plasmid
    • Primer
  • Supplier
    • antibodies-online
    • OriGene
    • GenScript
  • Reactivity
    • Human
    • Rat
    • Mouse
  • Application
    • Protein Expression (PExp)
    • Genome Editing with Engineered Nucleases (GEEN)
    • RNA Interference (RNAi)
    • Cloning (Clon)
  • Conjugate
    • Un-conjugated
    • GFP tag
    • His tag
    • Myc-DYKDDDDK Tag
    • HA tag
    • His-GST
    • His-MBP
    • DYKDDDDK Tag
    • RFP tag
  • Vector Backbone
    • pLenti-U6-sgRNA-PGK-Neo
    • pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro
    • pCMV6-Entry
    • pGFP-C-shLenti
    • pGFP-V-RS
    • pORF
    • pPB-His-GST
    • pPB-His-MBP
    • pPB-N-His
    • pPM-C-HA
    • pPM-C-His
    • pRS
    • pCas-Guide
    • pLenti-C-Myc-DDK
    • pLenti-C-mGFP
    • pCMV6-AC
    • pCMV6-AC-GFP
    • pCMV6-XL5
    • pRFP-C-RS
    • pcDNA3.1+C-(K)-DYK
  • Vector
    • Lentiviral Vector
    • Mammalian Expression Vector
    • Bacterial Expression Vector
    • Retroviral Vector
    • Cloning Vector
  • Insert
    • cDNA
    • ORF
    • shRNA
    • gRNA + Cas9
    • gRNA
  • Type of Oligonucleotide
    • siRNA Oligo
  • Bacterial Resistance
    • Ampicillin
    • Kanamycin
    • Chloramphenicol
    • Spectinomycin
  • Expression Type
    • Transient
    • Stable
  • Selectable Marker
    • Neomycin
    • Puromycin
  • Promoter
    • U6 Promoter
    • CMV Promoter
    • T7 Promoter
    • PGK Promoter
    • SFFV Promoter
    • Enhanced CMV Promoter
  • Format
    • Lyophilized
    • Liquid
    • Viral Particles

Synonyms and alternative names related to DBT

  • dihydrolipoamide branched chain transacylase E2 (DBT)
  • dihydrolipoamide branched chain transacylase E2 (dbt)
  • dihydrolipoamide branched chain transacylase E2 (CpipJ_CPIJ006326)
  • dihydrolipoamide branched chain transacylase E2 (BDBG_05874)
  • dihydrolipoamide branched chain transacylase E2 (NAEGRDRAFT_78509)
  • dihydrolipoamide branched chain transacylase E2 (VDBG_04820)
  • hypothetical protein (PGTG_17722)
  • dihydrolipoamide branched chain transacylase E2 (Dbt)
  • dihydrolipoamide branched chain transacylase E2 L homeolog (dbt.L)
  • BCATE2
  • BCKAD-E2
  • BCKADE2
  • D3Wsu60e
  • E2
  • E2B
  • E2b
  • im:7147214
  • zgc:103768

Gene-IDs for different species

Protein level used designations for DBT

  • lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial
  • dihydrolipoamide branched chain transacylase E2
  • BCKAD E2 subunit
  • E2 component of branched chain alpha-keto acid dehydrogenase complex
  • branched chain acyltransferase, E2 component
  • branched-chain alpha-keto acid dehydrogenase complex component E2
  • dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex
  • dihydrolipoyl transacylase
  • dihydrolipoyllysine-residue (2-methylpropanoyl)transferase
  • lipoamide acyltransferase component of mitochondrial branched-chain alpha-keto acid dehydrogenase complex
  • mitochondrial branched chain alpha-keto acid dehydrogenase transacylase subunit (E2b)
  • BCKAD E2
  • BCKAD-E2
  • BCKADE2
  • branched-chain alpha-ketoacid dehydrogenase, E2 subunit
  • part of the BCKAD complex
  • que
  • mitochondrial branched-chain alpha-keto acid dihydrolipoyl acyltransferase
  • component of branched chain keto acid dehydrogenase complex
Other products related to DBT such as antibodies, ELISA kits and high-purity proteins are available on our partner website antibodies-online.com
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