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GRLF1 (Glucocorticoid Receptor DNA Binding Factor 1, GRLF1)

Short Description: The human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. The amino acid sequence deduced from the cDNA sequences show the presence of three sequence motifs characteristic of a zinc finger and one motif suggestive of a leucine zipper in which 1 cysteine is found instead of all leucines. The GRLF1 enhances the homologous down-regulation of wild-type hGR gene expression. Biochemical analysis suggests that GRLF1 interaction is sequence specific and that transcriptional efficacy of GRLF1 is regulated through its interaction with specific sequence motif. The level of expression is regulated by glucocorticoids. [provided by RefSeq, Jul 2008].
More information related to gene GRLF1.

Products related to GRLF1 Gene:

36 results :

Human GRLF1 cDNA Clone in Mammalian Expression Vector (HA tag)
cDNA PExp Mammalian Expression Vector pPM-C-HA Kanamycin CMV Promoter HA tag Stable
Type:
cDNA
Application:
Protein Expression
Gene:
Glucocorticoid Receptor DNA Binding Factor 1 (GRLF1)
Species:
Human
Vector type:
Mammalian Expression Vector
Vector backbone:
pPM-C-HA
Resistance Gene:
Kanamycin
Selection Marker:
Neomycin
Promoter:
CMV Promoter
Fusion Tag:
HA tag
Expression:
Stable, Transient
Catalog No. ABIN4476280
Datasheet
Catalog No. ABIN4476280
Type:
cDNA
Application:
Protein Expression
Gene:
Glucocorticoid Receptor DNA Binding Factor 1 (GRLF1)
Species:
Human
Synonyms:
ARHGAP35, Arhgap35, arhgap35.L
Insert length:
111 bp
Vector type:
Mammalian Expression Vector
Vector backbone:
pPM-C-HA
Resistance Gene:
Kanamycin
Selection Marker:
Neomycin
Promoter:
CMV Promoter
Fusion Tag:
HA tag
Expression:
Stable, Transient
Sequencing Primer:
  • CMV sequencing primer: 5` CGC AAA TGG GCG GTA GGC GTG 3`
Format:
Liquid
Buffer:
10 mM Tris-HCI, 1 mM EDTA, pH 8.0
Storage:
-20 °C
Mouse GRLF1 CRISPR gRNA in Lenti Particles
gRNA PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-PGK-Neo Ampicillin U6 Promoter unconjugated Stable
Type:
gRNA
Application:
Protein Expression, Genome Editing with Engineered Nucleases
Gene:
Glucocorticoid Receptor DNA Binding Factor 1 (GRLF1)
NCBI Accession:
NM_172739
Species:
Mouse
Vector type:
Lentiviral Vector
Vector backbone:
pLenti-U6-sgRNA-PGK-Neo
Resistance Gene:
Ampicillin
Selection Marker:
Neomycin
Promoter:
U6 Promoter, PGK Promoter
Expression:
Stable, Transient
Catalog No. ABIN5089616
Datasheet
Catalog No. ABIN5089616
Type:
gRNA
Application:
Protein Expression, Genome Editing with Engineered Nucleases
Gene:
Glucocorticoid Receptor DNA Binding Factor 1 (GRLF1)
NCBI Accession:
NM_172739
Species:
Mouse
Synonyms:
ARHGAP35, Arhgap35, arhgap35.L
Vector type:
Lentiviral Vector
Vector backbone:
pLenti-U6-sgRNA-PGK-Neo
Resistance Gene:
Ampicillin
Selection Marker:
Neomycin
Promoter:
U6 Promoter, PGK Promoter
Expression:
Stable, Transient
Components:
Lentiviral particles with an individual gRNA (300 μL) for a specific sequence of Grlf1
Format:
Viral Particles
Storage:
-80 °C
Human GRLF1 CRISPR gRNA in Lenti Particles
gRNA PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-PGK-Neo Ampicillin U6 Promoter unconjugated Stable
Type:
gRNA
Application:
Protein Expression, Genome Editing with Engineered Nucleases
Gene:
Glucocorticoid Receptor DNA Binding Factor 1 (GRLF1)
NCBI Accession:
NM_004491
Species:
Human
Vector type:
Lentiviral Vector
Vector backbone:
pLenti-U6-sgRNA-PGK-Neo
Resistance Gene:
Ampicillin
Selection Marker:
Neomycin
Promoter:
U6 Promoter, PGK Promoter
Expression:
Stable, Transient
Catalog No. ABIN5089614
Datasheet
Catalog No. ABIN5089614
Type:
gRNA
Application:
Protein Expression, Genome Editing with Engineered Nucleases
Gene:
Glucocorticoid Receptor DNA Binding Factor 1 (GRLF1)
NCBI Accession:
NM_004491
Species:
Human
Synonyms:
ARHGAP35, Arhgap35, arhgap35.L
Vector type:
Lentiviral Vector
Vector backbone:
pLenti-U6-sgRNA-PGK-Neo
Resistance Gene:
Ampicillin
Selection Marker:
Neomycin
Promoter:
U6 Promoter, PGK Promoter
Expression:
Stable, Transient
Components:
Lentiviral particles with an individual gRNA (300 μL) for a specific sequence of GRLF1
Format:
Viral Particles
Storage:
-80 °C
Human GRLF1 cDNA Clone in Bacterial Expression Vector (His tag)
cDNA Clon Bacterial Expression Vector pPB-N-His Kanamycin T7 Promoter His tag Transient
Type:
cDNA
Application:
Cloning
Gene:
Glucocorticoid Receptor DNA Binding Factor 1 (GRLF1)
Species:
Human
Vector type:
Bacterial Expression Vector
Vector backbone:
pPB-N-His
Resistance Gene:
Kanamycin
Promoter:
T7 Promoter
Fusion Tag:
His tag
Expression:
Transient
Catalog No. ABIN4762655
Datasheet
Catalog No. ABIN4762655
Type:
cDNA
Application:
Cloning
Gene:
Glucocorticoid Receptor DNA Binding Factor 1 (GRLF1)
Species:
Human
Synonyms:
ARHGAP35, Arhgap35, arhgap35.L
Insert length:
111 bp
Vector type:
Bacterial Expression Vector
Vector backbone:
pPB-N-His
Resistance Gene:
Kanamycin
Promoter:
T7 Promoter
Fusion Tag:
His tag
Expression:
Transient
Sequencing Primer:
  • T7 promoter primer: 5'-TAATACGACTCACTATAGGG-3'
  • T7 terminator primer: 5'-GCTAGTTATTGCTCAGCGG-3'
Format:
Liquid
Buffer:
10 mM Tris-HCI, 1 mM EDTA, pH 8.0
Storage:
-20 °C
Human GRLF1 cDNA Clone in Mammalian Expression Vector (His tag)
cDNA PExp Mammalian Expression Vector pPM-C-His Kanamycin CMV Promoter His tag Stable
Type:
cDNA
Application:
Protein Expression
Gene:
Glucocorticoid Receptor DNA Binding Factor 1 (GRLF1)
Species:
Human
Vector type:
Mammalian Expression Vector
Vector backbone:
pPM-C-His
Resistance Gene:
Kanamycin
Selection Marker:
Neomycin
Promoter:
CMV Promoter
Fusion Tag:
His tag
Expression:
Stable, Transient
Catalog No. ABIN4437375
Datasheet
Catalog No. ABIN4437375
Type:
cDNA
Application:
Protein Expression
Gene:
Glucocorticoid Receptor DNA Binding Factor 1 (GRLF1)
Species:
Human
Synonyms:
ARHGAP35, Arhgap35, arhgap35.L
Insert length:
111 bp
Vector type:
Mammalian Expression Vector
Vector backbone:
pPM-C-His
Resistance Gene:
Kanamycin
Selection Marker:
Neomycin
Promoter:
CMV Promoter
Fusion Tag:
His tag
Expression:
Stable, Transient
Sequencing Primer:
  • CMV sequencing primer: 5` CGC AAA TGG GCG GTA GGC GTG 3`
Format:
Liquid
Buffer:
10 mM Tris-HCI, 1 mM EDTA, pH 8.0
Storage:
-20 °C
Human GRLF1 CRISPR gRNA + Cas9 in Lenti Particles
gRNA + Cas9 PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro Ampicillin U6 Promoter unconjugated Stable
Type:
gRNA + Cas9
Application:
Protein Expression, Genome Editing with Engineered Nucleases
Gene:
Glucocorticoid Receptor DNA Binding Factor 1 (GRLF1)
NCBI Accession:
NM_004491
Species:
Human
Vector type:
Lentiviral Vector
Resistance Gene:
Ampicillin
Selection Marker:
Puromycin
Promoter:
U6 Promoter, SFFV Promoter
Expression:
Stable, Transient
Catalog No. ABIN5204774
Datasheet
Catalog No. ABIN5204774
Type:
gRNA + Cas9
Application:
Protein Expression, Genome Editing with Engineered Nucleases
Gene:
Glucocorticoid Receptor DNA Binding Factor 1 (GRLF1)
NCBI Accession:
NM_004491
Species:
Human
Synonyms:
ARHGAP35, Arhgap35, arhgap35.L
Vector type:
Lentiviral Vector
Vector backbone:
pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro
Resistance Gene:
Ampicillin
Selection Marker:
Puromycin
Promoter:
U6 Promoter, SFFV Promoter
Expression:
Stable, Transient
Sequencing Primer:
  • U6 Forward Primer: 5'--TACGTCCAAGGTCGGGCAGGAAGA--3'
Components:
Lentiviral particles with an individual gRNA (300 μL) for a specific sequence of GRLF1
Format:
Viral Particles
Storage:
-80 °C
Mouse GRLF1 CRISPR gRNA + Cas9 in Lenti Particles
gRNA + Cas9 PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro Ampicillin U6 Promoter unconjugated Stable
Type:
gRNA + Cas9
Application:
Protein Expression, Genome Editing with Engineered Nucleases
Gene:
Glucocorticoid Receptor DNA Binding Factor 1 (GRLF1)
NCBI Accession:
NM_172739
Species:
Mouse
Vector type:
Lentiviral Vector
Resistance Gene:
Ampicillin
Selection Marker:
Puromycin
Promoter:
U6 Promoter, SFFV Promoter
Expression:
Stable, Transient
Catalog No. ABIN5204776
Datasheet
Catalog No. ABIN5204776
Type:
gRNA + Cas9
Application:
Protein Expression, Genome Editing with Engineered Nucleases
Gene:
Glucocorticoid Receptor DNA Binding Factor 1 (GRLF1)
NCBI Accession:
NM_172739
Species:
Mouse
Synonyms:
ARHGAP35, Arhgap35, arhgap35.L
Vector type:
Lentiviral Vector
Vector backbone:
pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro
Resistance Gene:
Ampicillin
Selection Marker:
Puromycin
Promoter:
U6 Promoter, SFFV Promoter
Expression:
Stable, Transient
Sequencing Primer:
  • U6 Forward Primer: 5'--TACGTCCAAGGTCGGGCAGGAAGA--3'
Components:
Lentiviral particles with an individual gRNA (300 μL) for a specific sequence of Grlf1
Format:
Viral Particles
Storage:
-80 °C
Mouse GRLF1 CRISPR gRNA in Lenti Particles
gRNA PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-PGK-Neo Ampicillin U6 Promoter unconjugated Stable
Type:
gRNA
Application:
Protein Expression, Genome Editing with Engineered Nucleases
Gene:
Glucocorticoid Receptor DNA Binding Factor 1 (GRLF1)
NCBI Accession:
NM_172739
Species:
Mouse
Vector type:
Lentiviral Vector
Vector backbone:
pLenti-U6-sgRNA-PGK-Neo
Resistance Gene:
Ampicillin
Selection Marker:
Neomycin
Promoter:
U6 Promoter, PGK Promoter
Expression:
Stable, Transient
Catalog No. ABIN5089615
Datasheet
Catalog No. ABIN5089615
Type:
gRNA
Application:
Protein Expression, Genome Editing with Engineered Nucleases
Gene:
Glucocorticoid Receptor DNA Binding Factor 1 (GRLF1)
NCBI Accession:
NM_172739
Species:
Mouse
Synonyms:
ARHGAP35, Arhgap35, arhgap35.L
Vector type:
Lentiviral Vector
Vector backbone:
pLenti-U6-sgRNA-PGK-Neo
Resistance Gene:
Ampicillin
Selection Marker:
Neomycin
Promoter:
U6 Promoter, PGK Promoter
Expression:
Stable, Transient
Components:
Lentiviral particles with a set of 3 gRNAs (3 x 300 μL) covering different sequences of Grlf1
Format:
Viral Particles
Storage:
-80 °C
Human GRLF1 CRISPR gRNA in Lenti Particles
gRNA PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-PGK-Neo Ampicillin U6 Promoter unconjugated Stable
Type:
gRNA
Application:
Protein Expression, Genome Editing with Engineered Nucleases
Gene:
Glucocorticoid Receptor DNA Binding Factor 1 (GRLF1)
NCBI Accession:
NM_004491
Species:
Human
Vector type:
Lentiviral Vector
Vector backbone:
pLenti-U6-sgRNA-PGK-Neo
Resistance Gene:
Ampicillin
Selection Marker:
Neomycin
Promoter:
U6 Promoter, PGK Promoter
Expression:
Stable, Transient
Catalog No. ABIN5089613
Datasheet
Catalog No. ABIN5089613
Type:
gRNA
Application:
Protein Expression, Genome Editing with Engineered Nucleases
Gene:
Glucocorticoid Receptor DNA Binding Factor 1 (GRLF1)
NCBI Accession:
NM_004491
Species:
Human
Synonyms:
ARHGAP35, Arhgap35, arhgap35.L
Vector type:
Lentiviral Vector
Vector backbone:
pLenti-U6-sgRNA-PGK-Neo
Resistance Gene:
Ampicillin
Selection Marker:
Neomycin
Promoter:
U6 Promoter, PGK Promoter
Expression:
Stable, Transient
Components:
Lentiviral particles with a set of 3 gRNAs (3 x 300 μL) covering different sequences of GRLF1
Format:
Viral Particles
Storage:
-80 °C
Human GRLF1 CRISPR gRNA + Cas9 in Lenti Particles
gRNA + Cas9 PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro Ampicillin U6 Promoter unconjugated Stable
Type:
gRNA + Cas9
Application:
Protein Expression, Genome Editing with Engineered Nucleases
Gene:
Glucocorticoid Receptor DNA Binding Factor 1 (GRLF1)
NCBI Accession:
NM_004491
Species:
Human
Vector type:
Lentiviral Vector
Resistance Gene:
Ampicillin
Selection Marker:
Puromycin
Promoter:
U6 Promoter, SFFV Promoter
Expression:
Stable, Transient
Catalog No. ABIN5204773
Datasheet
Catalog No. ABIN5204773
Type:
gRNA + Cas9
Application:
Protein Expression, Genome Editing with Engineered Nucleases
Gene:
Glucocorticoid Receptor DNA Binding Factor 1 (GRLF1)
NCBI Accession:
NM_004491
Species:
Human
Synonyms:
ARHGAP35, Arhgap35, arhgap35.L
Vector type:
Lentiviral Vector
Vector backbone:
pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro
Resistance Gene:
Ampicillin
Selection Marker:
Puromycin
Promoter:
U6 Promoter, SFFV Promoter
Expression:
Stable, Transient
Sequencing Primer:
  • U6 Forward Primer: 5'--TACGTCCAAGGTCGGGCAGGAAGA--3'
Components:
Lentiviral particles with a set of 3 gRNAs (3 x 300 μL) covering different sequences of GRLF1
Format:
Viral Particles
Storage:
-80 °C
  • Type
    • Plasmid
    • Primer
  • Supplier
    • OriGene
    • antibodies-online
    • GenScript
    • ProteoGenix
  • Reactivity
    • Human
    • Mouse
    • Rat
  • Application
    • Protein Expression (PExp)
    • Genome Editing with Engineered Nucleases (GEEN)
    • RNA Interference (RNAi)
    • Cloning (Clon)
  • Conjugate
    • Un-conjugated
    • GFP tag
    • His tag
    • Myc-DYKDDDDK Tag
    • HA tag
    • DYKDDDDK Tag
    • His-GST
    • His-MBP
    • RFP tag
  • Vector Backbone
    • pCMV6-Entry
    • pLenti-U6-sgRNA-PGK-Neo
    • pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro
    • pCas-Guide
    • pGFP-C-shLenti
    • pGFP-V-RS
    • pPB-N-His
    • pPM-C-HA
    • pPM-C-His
    • pRS
    • pCMV6-AC-GFP
    • pDONR223
    • pORF
    • pPB-His-GST
    • pPB-His-MBP
    • pRFP-C-RS
    • pcDNA3.1+C-(K)-DYK
  • Vector
    • Mammalian Expression Vector
    • Lentiviral Vector
    • Retroviral Vector
    • Bacterial Expression Vector
    • Cloning Vector
    • Gateway Cloning Vector
  • Insert
    • cDNA
    • shRNA
    • ORF
    • gRNA + Cas9
    • gRNA
  • Type of Oligonucleotide
    • siRNA Oligo
  • Bacterial Resistance
    • Kanamycin
    • Ampicillin
    • Chloramphenicol
    • Spectinomycin
  • Expression Type
    • Transient
    • Stable
  • Selectable Marker
    • Neomycin
    • Puromycin
  • Promoter
    • U6 Promoter
    • CMV Promoter
    • Enhanced CMV Promoter
    • PGK Promoter
    • SFFV Promoter
    • T7 Promoter
  • Format
    • Lyophilized
    • Liquid
    • Viral Particles

Synonyms and alternative names related to GRLF1

  • Rho GTPase activating protein 35 (ARHGAP35)
  • Rho GTPase activating protein 35 (Arhgap35)
  • Rho GTPase activating protein 35 L homeolog (arhgap35.L)
  • 6430596G11Rik
  • AI841135
  • Arhgap35
  • arhgap35
  • GRF-1
  • grf-1
  • GRLF1
  • Grlf1
  • grlf1
  • mKIAA1722
  • P190-A
  • p190-A
  • p190-a
  • P190A
  • p190A
  • p190a
  • p190ARhoGAP
  • p190arhogap
  • p190RhoGAP
  • p190rhogap

Gene-IDs for different species

Protein level used designations for GRLF1

  • glucocorticoid receptor DNA binding factor 1
  • glucocorticoid receptor DNA-binding factor 1
  • glucocorticoid receptor repression factor 1
  • rho GAP p190A
  • rho GTPase-activating protein 35
  • P190 RhoGAP
  • GAP-associated protein p190
  • Rho GTPase activating protein 35 L homeolog
  • rho GTPase activating protein 35 L homeolog
Other products related to GRLF1 such as antibodies, ELISA kits and high-purity proteins are available on our partner website antibodies-online.com
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