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RPE65 (Retinal Pigment Epithelium-Specific Protein 65kDa, RPE65)

Short Description: This gene encodes a protein which is located in the retinal pigment epithelium and is involved in the production of 11-cis retinal and in visual pigment regeneration. There are two forms of this protein, a soluble form called sRPE65, and a palmitoylated, membrane-bound form known as mRPE65. mRPE65 serves as the palmitoyl donor for lecithin retinol acyl transferase (LRAT), the enzyme that catalyzes the vitamin A to all trans retinol step of the chromophore regeneration process. Both mRPE65 and sRPE65 also serve as regulatory proteins, with the ratio and concentrations of these molecules playing a role in the inhibition of 11-cis retinal synthesis. Mutations in this gene have been associated with Leber congenital amaurosis type 2 (LCA2) and retinitis pigmentosa. [provided by RefSeq, Jul 2008].
More information related to gene RPE65.

Products related to RPE65 Gene:

55 results :

Human RPE65 ORF Clone in Cloning Vector
ORF Clon Cloning Vector pORF Spectinomycin unconjugated Transient
Type:
ORF
Application:
Cloning
Gene:
Retinal Pigment Epithelium-Specific Protein 65kDa (RPE65)
NCBI Accession:
NM_000329
Species:
Human
Vector type:
Cloning Vector
Vector backbone:
pORF
Resistance Gene:
Spectinomycin
Expression:
Transient
Catalog No. ABIN5742277
Datasheet
Catalog No. ABIN5742277
Type:
ORF
Application:
Cloning
Gene:
Retinal Pigment Epithelium-Specific Protein 65kDa (RPE65)
NCBI Accession:
NM_000329
Species:
Human
Synonyms:
RPE65, Rpe65, ninaB, rpe65.S, rpe65.L, rpe65, rpe65c, rpe65b, rpe65a
Insert length:
1602 bp
Vector type:
Cloning Vector
Vector backbone:
pORF
Resistance Gene:
Spectinomycin
Expression:
Transient
Sequencing Primer:
  • M13 FP: 5'-CCCAGTCACGACGTTGTAAAACG-3'
    M13 RP: 5'-CAGGAAACAGCTATGAC-3'
Format:
Liquid
Buffer:
10  mM Tris-HCI, 1  mM EDTA,  pH 8.0
Storage:
-20 °C
Human RPE65 cDNA Clone in Mammalian Expression Vector (HA tag)
cDNA PExp Mammalian Expression Vector pPM-C-HA Kanamycin CMV Promoter HA tag Stable
Type:
cDNA
Application:
Protein Expression
Gene:
Retinal Pigment Epithelium-Specific Protein 65kDa (RPE65)
NCBI Accession:
NM_000329
Species:
Human
Vector type:
Mammalian Expression Vector
Vector backbone:
pPM-C-HA
Resistance Gene:
Kanamycin
Selection Marker:
Neomycin
Promoter:
CMV Promoter
Fusion Tag:
HA tag
Expression:
Stable, Transient
Catalog No. ABIN4490127
Datasheet
Catalog No. ABIN4490127
Type:
cDNA
Application:
Protein Expression
Gene:
Retinal Pigment Epithelium-Specific Protein 65kDa (RPE65)
NCBI Accession:
NM_000329
Species:
Human
Synonyms:
RPE65, Rpe65, ninaB, rpe65.S, rpe65.L, rpe65, rpe65c, rpe65b, rpe65a
Insert length:
1602 bp
Vector type:
Mammalian Expression Vector
Vector backbone:
pPM-C-HA
Resistance Gene:
Kanamycin
Selection Marker:
Neomycin
Promoter:
CMV Promoter
Fusion Tag:
HA tag
Expression:
Stable, Transient
Sequencing Primer:
  • CMV sequencing primer: 5` CGC AAA TGG GCG GTA GGC GTG 3`
Format:
Liquid
Buffer:
10 mM Tris-HCI, 1 mM EDTA, pH 8.0
Storage:
-20 °C
Rat RPE65 CRISPR gRNA in Lenti Particles
gRNA PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-PGK-Neo Ampicillin U6 Promoter unconjugated Stable
Type:
gRNA
Application:
Protein Expression, Genome Editing with Engineered Nucleases
Gene:
Retinal Pigment Epithelium-Specific Protein 65kDa (RPE65)
NCBI Accession:
NM_053562
Species:
Rat
Vector type:
Lentiviral Vector
Vector backbone:
pLenti-U6-sgRNA-PGK-Neo
Resistance Gene:
Ampicillin
Selection Marker:
Neomycin
Promoter:
U6 Promoter, PGK Promoter
Expression:
Stable, Transient
Catalog No. ABIN5122136
Datasheet
Catalog No. ABIN5122136
Type:
gRNA
Application:
Protein Expression, Genome Editing with Engineered Nucleases
Gene:
Retinal Pigment Epithelium-Specific Protein 65kDa (RPE65)
NCBI Accession:
NM_053562
Species:
Rat
Synonyms:
RPE65, Rpe65, ninaB, rpe65.S, rpe65.L, rpe65, rpe65c, rpe65b, rpe65a
Vector type:
Lentiviral Vector
Vector backbone:
pLenti-U6-sgRNA-PGK-Neo
Resistance Gene:
Ampicillin
Selection Marker:
Neomycin
Promoter:
U6 Promoter, PGK Promoter
Expression:
Stable, Transient
Components:
Lentiviral particles with an individual gRNA (300 μL) for a specific sequence of Rpe65
Format:
Viral Particles
Storage:
-80 °C
Mouse RPE65 CRISPR gRNA in Lenti Particles
gRNA PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-PGK-Neo Ampicillin U6 Promoter unconjugated Stable
Type:
gRNA
Application:
Protein Expression, Genome Editing with Engineered Nucleases
Gene:
Retinal Pigment Epithelium-Specific Protein 65kDa (RPE65)
NCBI Accession:
NM_029987
Species:
Mouse
Vector type:
Lentiviral Vector
Vector backbone:
pLenti-U6-sgRNA-PGK-Neo
Resistance Gene:
Ampicillin
Selection Marker:
Neomycin
Promoter:
U6 Promoter, PGK Promoter
Expression:
Stable, Transient
Catalog No. ABIN5122134
Datasheet
Catalog No. ABIN5122134
Type:
gRNA
Application:
Protein Expression, Genome Editing with Engineered Nucleases
Gene:
Retinal Pigment Epithelium-Specific Protein 65kDa (RPE65)
NCBI Accession:
NM_029987
Species:
Mouse
Synonyms:
RPE65, Rpe65, ninaB, rpe65.S, rpe65.L, rpe65, rpe65c, rpe65b, rpe65a
Vector type:
Lentiviral Vector
Vector backbone:
pLenti-U6-sgRNA-PGK-Neo
Resistance Gene:
Ampicillin
Selection Marker:
Neomycin
Promoter:
U6 Promoter, PGK Promoter
Expression:
Stable, Transient
Components:
Lentiviral particles with an individual gRNA (300 μL) for a specific sequence of Rpe65
Format:
Viral Particles
Storage:
-80 °C
Human RPE65 CRISPR gRNA in Lenti Particles
gRNA PExp, GEEN Lentiviral Vector pLenti-U6-sgRNA-PGK-Neo Ampicillin U6 Promoter unconjugated Stable
Type:
gRNA
Application:
Protein Expression, Genome Editing with Engineered Nucleases
Gene:
Retinal Pigment Epithelium-Specific Protein 65kDa (RPE65)
NCBI Accession:
NM_000329
Species:
Human
Vector type:
Lentiviral Vector
Vector backbone:
pLenti-U6-sgRNA-PGK-Neo
Resistance Gene:
Ampicillin
Selection Marker:
Neomycin
Promoter:
U6 Promoter, PGK Promoter
Expression:
Stable, Transient
Catalog No. ABIN5122132
Datasheet
Catalog No. ABIN5122132
Type:
gRNA
Application:
Protein Expression, Genome Editing with Engineered Nucleases
Gene:
Retinal Pigment Epithelium-Specific Protein 65kDa (RPE65)
NCBI Accession:
NM_000329
Species:
Human
Synonyms:
RPE65, Rpe65, ninaB, rpe65.S, rpe65.L, rpe65, rpe65c, rpe65b, rpe65a
Vector type:
Lentiviral Vector
Vector backbone:
pLenti-U6-sgRNA-PGK-Neo
Resistance Gene:
Ampicillin
Selection Marker:
Neomycin
Promoter:
U6 Promoter, PGK Promoter
Expression:
Stable, Transient
Components:
Lentiviral particles with an individual gRNA (300 μL) for a specific sequence of RPE65
Format:
Viral Particles
Storage:
-80 °C
Human RPE65 cDNA Clone in Bacterial Expression Vector (His-GST)
cDNA Clon Bacterial Expression Vector pPB-His-GST Kanamycin T7 Promoter His-GST Transient
Type:
cDNA
Application:
Cloning
Gene:
Retinal Pigment Epithelium-Specific Protein 65kDa (RPE65)
NCBI Accession:
NM_000329
Species:
Human
Vector type:
Bacterial Expression Vector
Vector backbone:
pPB-His-GST
Resistance Gene:
Kanamycin
Promoter:
T7 Promoter
Fusion Tag:
His-GST
Expression:
Transient
Catalog No. ABIN4839262
Datasheet
Catalog No. ABIN4839262
Type:
cDNA
Application:
Cloning
Gene:
Retinal Pigment Epithelium-Specific Protein 65kDa (RPE65)
NCBI Accession:
NM_000329
Species:
Human
Synonyms:
RPE65, Rpe65, ninaB, rpe65.S, rpe65.L, rpe65, rpe65c, rpe65b, rpe65a
Insert length:
1602 bp
Vector type:
Bacterial Expression Vector
Vector backbone:
pPB-His-GST
Resistance Gene:
Kanamycin
Promoter:
T7 Promoter
Fusion Tag:
His-GST
Expression:
Transient
Sequencing Primer:
  • GST Forward primer: 5'-CACGTTTGGTGGTGGCGAC3'
  • T7 terminator primer: 5'-GCTAGTTATTGCTCAGCGG-3'
Format:
Liquid
Buffer:
10 mM Tris-HCI, 1 mM EDTA, pH 8.0
Storage:
-20 °C
Human RPE65 cDNA Clone in Bacterial Expression Vector (His tag)
cDNA Clon Bacterial Expression Vector pPB-N-His Kanamycin T7 Promoter His tag Transient
Type:
cDNA
Application:
Cloning
Gene:
Retinal Pigment Epithelium-Specific Protein 65kDa (RPE65)
NCBI Accession:
NM_000329
Species:
Human
Vector type:
Bacterial Expression Vector
Vector backbone:
pPB-N-His
Resistance Gene:
Kanamycin
Promoter:
T7 Promoter
Fusion Tag:
His tag
Expression:
Transient
Catalog No. ABIN4633596
Datasheet
Catalog No. ABIN4633596
Type:
cDNA
Application:
Cloning
Gene:
Retinal Pigment Epithelium-Specific Protein 65kDa (RPE65)
NCBI Accession:
NM_000329
Species:
Human
Synonyms:
RPE65, Rpe65, ninaB, rpe65.S, rpe65.L, rpe65, rpe65c, rpe65b, rpe65a
Insert length:
1602 bp
Vector type:
Bacterial Expression Vector
Vector backbone:
pPB-N-His
Resistance Gene:
Kanamycin
Promoter:
T7 Promoter
Fusion Tag:
His tag
Expression:
Transient
Sequencing Primer:
  • T7 promoter primer: 5'-TAATACGACTCACTATAGGG-3'
  • T7 terminator primer: 5'-GCTAGTTATTGCTCAGCGG-3'
Format:
Liquid
Buffer:
10 mM Tris-HCI, 1 mM EDTA, pH 8.0
Storage:
-20 °C
Human RPE65 cDNA Clone in Bacterial Expression Vector (His-MBP)
cDNA Clon Bacterial Expression Vector pPB-His-MBP Kanamycin T7 Promoter His-MBP Transient
Type:
cDNA
Application:
Cloning
Gene:
Retinal Pigment Epithelium-Specific Protein 65kDa (RPE65)
NCBI Accession:
NM_000329
Species:
Human
Vector type:
Bacterial Expression Vector
Vector backbone:
pPB-His-MBP
Resistance Gene:
Kanamycin
Promoter:
T7 Promoter
Fusion Tag:
His-MBP
Expression:
Transient
Catalog No. ABIN4710131
Datasheet
Catalog No. ABIN4710131
Type:
cDNA
Application:
Cloning
Gene:
Retinal Pigment Epithelium-Specific Protein 65kDa (RPE65)
NCBI Accession:
NM_000329
Species:
Human
Synonyms:
RPE65, Rpe65, ninaB, rpe65.S, rpe65.L, rpe65, rpe65c, rpe65b, rpe65a
Insert length:
1602 bp
Vector type:
Bacterial Expression Vector
Vector backbone:
pPB-His-MBP
Resistance Gene:
Kanamycin
Promoter:
T7 Promoter
Fusion Tag:
His-MBP
Expression:
Transient
Sequencing Primer:
  • MBP Forward primer: 5'-CGCAGATGTCCGCTTTCTGG-3'
  • T7 terminator primer: 5'-GCTAGTTATTGCTCAGCGG-3'
Format:
Liquid
Buffer:
10 mM Tris-HCI, 1 mM EDTA, pH 8.0
Storage:
-20 °C
Human RPE65 cDNA Clone in Mammalian Expression Vector (His tag)
cDNA PExp Mammalian Expression Vector pPM-C-His Kanamycin CMV Promoter His tag Stable
Type:
cDNA
Application:
Protein Expression
Gene:
Retinal Pigment Epithelium-Specific Protein 65kDa (RPE65)
NCBI Accession:
NM_000329
Species:
Human
Vector type:
Mammalian Expression Vector
Vector backbone:
pPM-C-His
Resistance Gene:
Kanamycin
Selection Marker:
Neomycin
Promoter:
CMV Promoter
Fusion Tag:
His tag
Expression:
Stable, Transient
Catalog No. ABIN4425037
Datasheet
Catalog No. ABIN4425037
Type:
cDNA
Application:
Protein Expression
Gene:
Retinal Pigment Epithelium-Specific Protein 65kDa (RPE65)
NCBI Accession:
NM_000329
Species:
Human
Synonyms:
RPE65, Rpe65, ninaB, rpe65.S, rpe65.L, rpe65, rpe65c, rpe65b, rpe65a
Insert length:
1602 bp
Vector type:
Mammalian Expression Vector
Vector backbone:
pPM-C-His
Resistance Gene:
Kanamycin
Selection Marker:
Neomycin
Promoter:
CMV Promoter
Fusion Tag:
His tag
Expression:
Stable, Transient
Sequencing Primer:
  • CMV sequencing primer: 5` CGC AAA TGG GCG GTA GGC GTG 3`
Format:
Liquid
Buffer:
10 mM Tris-HCI, 1 mM EDTA, pH 8.0
Storage:
-20 °C
Rat RPE65 ORF Clone in Cloning Vector
ORF Clon Cloning Vector pORF Spectinomycin unconjugated Transient
Type:
ORF
Application:
Cloning
Gene:
Retinal Pigment Epithelium-Specific Protein 65kDa (RPE65)
NCBI Accession:
NM_053562
Species:
Rat
Vector type:
Cloning Vector
Vector backbone:
pORF
Resistance Gene:
Spectinomycin
Expression:
Transient
Catalog No. ABIN5742279
Datasheet
Catalog No. ABIN5742279
Type:
ORF
Application:
Cloning
Gene:
Retinal Pigment Epithelium-Specific Protein 65kDa (RPE65)
NCBI Accession:
NM_053562
Species:
Rat
Synonyms:
RPE65, Rpe65, ninaB, rpe65.S, rpe65.L, rpe65, rpe65c, rpe65b, rpe65a
Insert length:
1602 bp
Vector type:
Cloning Vector
Vector backbone:
pORF
Resistance Gene:
Spectinomycin
Expression:
Transient
Sequencing Primer:
  • M13 FP: 5'-CCCAGTCACGACGTTGTAAAACG-3'
    M13 RP: 5'-CAGGAAACAGCTATGAC-3'
Format:
Liquid
Buffer:
10  mM Tris-HCI, 1  mM EDTA,  pH 8.0
Storage:
-20 °C
  • Type
    • Plasmid
    • Primer
  • Supplier
    • antibodies-online
    • OriGene
    • GenScript
  • Reactivity
    • Human
    • Mouse
    • Rat
  • Cited in publications
  • Application
    • Protein Expression (PExp)
    • RNA Interference (RNAi)
    • Genome Editing with Engineered Nucleases (GEEN)
    • Cloning (Clon)
  • Conjugate
    • Un-conjugated
    • GFP tag
    • His tag
    • Myc-DYKDDDDK Tag
    • HA tag
    • His-GST
    • His-MBP
    • RFP tag
    • DYKDDDDK Tag
  • Vector Backbone
    • pLenti-U6-sgRNA-PGK-Neo
    • pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro
    • pCMV6-Entry
    • pGFP-C-shLenti
    • pGFP-V-RS
    • pORF
    • pPB-His-GST
    • pPB-His-MBP
    • pPB-N-His
    • pPM-C-HA
    • pPM-C-His
    • pRS
    • pCas-Guide
    • pRFP-C-RS
    • pCMV6-AC-GFP
    • pCMV6-XL5
    • pLenti-C-Myc-DDK
    • pLenti-C-mGFP
    • pcDNA3.1+C-(K)-DYK
  • Vector
    • Lentiviral Vector
    • Mammalian Expression Vector
    • Bacterial Expression Vector
    • Retroviral Vector
    • Cloning Vector
  • Insert
    • cDNA
    • shRNA
    • ORF
    • gRNA + Cas9
    • gRNA
  • Type of Oligonucleotide
    • siRNA Oligo
  • Bacterial Resistance
    • Kanamycin
    • Ampicillin
    • Chloramphenicol
    • Spectinomycin
  • Expression Type
    • Transient
    • Stable
  • Selectable Marker
    • Puromycin
    • Neomycin
  • Promoter
    • U6 Promoter
    • CMV Promoter
    • T7 Promoter
    • PGK Promoter
    • SFFV Promoter
    • Enhanced CMV Promoter
  • Format
    • Lyophilized
    • Liquid
    • Viral Particles

Synonyms and alternative names related to RPE65

  • RPE65, retinoid isomerohydrolase (RPE65)
  • retinal pigment epithelium 65 (Rpe65)
  • neither inactivation nor afterpotential B (ninaB)
  • RPE65, retinoid isomerohydrolase (Rpe65)
  • retinal pigment epithelium-specific protein 65kDa S homeolog (rpe65.S)
  • retinal pigment epithelium-specific protein 65kDa L homeolog (rpe65.L)
  • RPE65 homolog (rpe65)
  • retinal pigment epithelium-specific protein 65kDa (rpe65)
  • retinal pigment epithelium-specific protein 65c (rpe65c)
  • retinal pigment epithelium-specific protein 65b (rpe65b)
  • retinal pigment epithelium-specific protein 65a (rpe65a)
  • 13cIMH
  • 65kDa
  • A930029L06Rik
  • anon-WO0148162.1
  • anon-WO0148163.1
  • beta-diox
  • CG9347
  • DmelCG9347
  • DRPE65
  • LCA2
  • MGC52887
  • MGC85437
  • MGC154296
  • Mord1
  • mRPE65
  • rd12
  • RP20
  • RPE56a
  • RPE56b
  • RPE56c
  • RPE65
  • rpe65
  • rpepa
  • rpepb
  • si:ch211-198n5.5
  • si:ch211-198n5.12
  • sRPE65
  • wu:fc74c09
  • zgc:73213
  • zgc:110538

Gene-IDs for different species

Protein level used designations for RPE65

  • RBP-binding membrane protein
  • all-trans-retinyl-palmitate hydrolase
  • p63
  • retinal pigment epithelium-specific 65 kDa protein
  • retinitis pigmentosa 20 (autosomal recessive)
  • retinoid isomerohydrolase
  • retinol isomerase
  • modifier of retinal degeneration 1
  • retinal pigment epithelium, 65 kDa
  • neither inactivation nor afterpotential B
  • CG9347-PA
  • ninaB-PA
  • beta-carotene dioxygenase
  • retinal pigment epithelium 65-like
  • retinal pigment epithelium-specific protein (65kD)
  • retinal pigment epithelium-specific protein 65kDa
  • retinal pigment epithelium abundant protein RPE65
  • rpe65 gene for retinal pigment epithelium-specific protein
  • RPE65 homolog
  • retinoid isomerohydrolase-like
  • All-trans-retinyl ester 13-cis isomerohydrolase C
  • All-trans-retinylester 11-cis isomerohydrolase C
  • Retinal pigment epithelium-specific 65 kDa protein homolog C
  • retinal Mueller cells isomerohydrolase
  • 13cIMH
  • Retinal pigment epithelium-specific 65 kDa protein homolog B
  • all-trans-retinyl ester 13-cis isomerohydrolase
  • retinal pigment epithelium 65b
  • retinal pigment epithelium-specific 65 kDa protein homolog B
  • retinal pigment epithelium-specific protein b
  • All-trans-retinylester 11-cis isomerohydrolase A
  • Retinal pigment epithelium-specific 65 kDa protein homolog A
  • all-trans-retinylester 11-cis isomerohydrolase A
  • retinal pigment epithelium 65a
  • retinal pigment epithelium-specific 65 kDa protein homolog A
  • retinal pigment epithelium-specific protein a
Other products related to RPE65 such as antibodies, ELISA kits and high-purity proteins are available on our partner website antibodies-online.com
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