GST-P1 PCR Assay
PCR
DNA
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- Application
- Polymerase Chain Reaction (PCR)
- Purpose
- MutaGEL® GST-P1 PCR Assay allows the detection of the Ile105Val polymorphism in the human glutathione-S-transferase gene (GST-P1).
- Brand
- MutaGEL®
- Sample Type
- DNA
- Characteristics
- The glutathione-S-transferase system protects against peroxides and electrophilic reaction partners which both can damage the cells of the body. The enzyme exists in several different isoforms. The solubility of the (manifold) substrates is increased by the addition of glutathione leading to a better elimination of the toxic metabolites from the body. Glutathione-S-transferase deficiency can be genetically determined. The GST-P1 gene compensates (at least in parts) for the frequently deletion of GST-M1 or GST-T1 genes in Caucasians (about 50% respectively 20%).
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- Comment
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MutaGEL GST-P1 PCR Assay kit contains a set of primers which amplify a specific sequence within the human GST-P1 gene (primer GST-P1). The amplified product obtained from a wild type DNA will not be cut by the restriction enzyme included in this kit, whereas the fragment obtained from DNA carrying the Il105Val-mutation will be cut once. The identification of the present genotype is done by analysis of the amplification products and their cut fragments through gel electrophoresis.
- Sample Volume
- 200 μL
- Restrictions
- For Research Use only
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- Storage
- -20 °C
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