EasyPure® Bacteria Genomic DNA Kit
PCR, RD
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- Application
- Polymerase Chain Reaction (PCR), Restriction Digest (RD)
- Purpose
- EasyPure® Bacteria Genomic DNA Kit uses lysozyme and moderate lysis buffer to lyse cells.
- Brand
- EasyPure®
- Characteristics
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Proteinase K is used for protein digestion and RNase A used for RNA digestion. DNA is specifically bound to silica-based column in hypersaline condition, and DNA is eluted by low salt and high pH solution. This kit is suitable for isolating high quality genomic DNA from Gram-positive and Gram-negative bacteria. The isolated DNA is suitable for PCR, restriction enzyme digestion, and Southern blot.
- Fast: the whole process can be completed in 50 minutes.
- High yield: DNA yield up to 20 μg. - Components
- Resuspension Buffer 11 (RB11), Lysis Buffer 11 (LB11), Binding Buffer 11 (BB11), Clean Buffer 11 (CB11), Wash Buffer 11 (WB11), Elution Buffer (EB), RNase A (10 mg/mL), Genomic Spin Columns with Collection Tubes
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- Application Notes
- PCR, restriction enzyme digestion, and Southern blot.
- Restrictions
- For Research Use only
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- Storage
- RT/-20 °C
- Storage Comment
- RNase A and Proteinase K solutions at -20°C for one year, others at room temperature for one year
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