EasyPure® Blood Genomic DNA Kit(No RNase A)
PCR, RD
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- Application
- Polymerase Chain Reaction (PCR), Restriction Digest (RD)
- Purpose
- EasyPure® Blood Genomic DNA Kit provides a simple and convenient way to isolate high quality genomic DNA from 5-250 μl of fresh or frozen blood.
- Brand
- EasyPure®
- Characteristics
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Whole blood is incubated with binding/lysis buffer to release DNA. DNA is bound to silica-based column.
- Simple and fast, red cell lysis buffer is no longer needed.
- Complete removal of contaminants and inhibitors.
- DNA yield up to 40 μg.
- Column based purification, no organic extraction or ethanol precipitation.
- Suitable for EDTA, sodium citrate and heparin-anticoagulated fresh or frozen blood in a volume of 5 to 250 μL. - Components
- Binding Buffer 3 (BB3), Clean Buffer 3 (CB3), Wash Buffer 3 (WB3), Elution Buffer (EB), Proteinase K (20 mg/mL), Genomic Spin Colums with Collection Tubes
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- Application Notes
- PCR, restriction enzyme digestion and Southern blot.
- Restrictions
- For Research Use only
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- Storage
- RT/-20 °C
- Storage Comment
- Proteinase K solutions at -20°C for one year, others at room temperature for one year
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