Vero Host Cell DNA Residue Detection Kit
qPCR
Species: Green Monkey
Quantitative
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- Gene
- Host Cell DNA
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Species
- Green Monkey
- Detection Range
- 1 fg/μL - 1000 pg/μL
- Minimum Detection Limit
- 1 fg/μL
- Application
- Quantitative real-time PCR (qPCR)
- Purpose
- The Vero Host Cell DNA Residue Detection Kit can be used for Quantitative analysis of DNA residue in recombinant protein expressed products, purified intermediate and finished products from the host cell.
- Analytical Method
- Quantitative
- Characteristics
- This kit adopts Taqman probe fluorescence qPCR method. The kit has the advantages of high specificity and sensitivity by using specific primers & probes, LOQ can reach 1fg/μL level. The preparation process of DNA Control is completely consistent with that of other host cell DNA national standards, therefore it has high purity and no protein and ion interference to ensure the accuracy of the sample quantitative detection. The kit provides DNA Dilution Buffer, which enables good replicate parallelism in a single experiment and good reproducibility between multiple experiments.
- Components
- 2XqPCR Mix, Primer&Probe Mix, DNA Dilution Buffer, DNA Control (10ng/μL), RNase-Free H2O, 50X ROX Reference Dye (Optional)
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Protocol
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- 2XqPCR Mix: 12.5 μL
- Primer&Probe Mix: 2 μL
- DNA template (control or sample): 5 μL
- Add water: 5.5 μL
- Total Volume: 25 μL
The detection range of the standard curve mentioned above is suitable for most experiments and can be adjusted as needed, such as 30fg/μL-300pg/μL. - Assay Precision
- Intra Variation% 4.8-12.5,Inter Variation% 14-17
- Restrictions
- For Research Use only
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- Storage
- -20 °C
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- Target
- Host Cell DNA
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