Cloning Kits

Molecular cloning of single or multiple inserts into plasmids for various purposes is an essential part in any lab work ranging from expression of recombinant proteins to large scale sequencing projects. Traditionally, DNA pieces of interest are first excised using restriction endonucleases and then stitched together via compatible ends using a ligase. More recently, restriction free cloning has been complemented by techniques like Gibson cloning in which a virtually unlimited number of fragments can be concatenated through recombination. Cloning kits provide all the necessary reagents in one complete package.