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Casilio Regulation

genomics-online offers plasmids with the casilio system. The casilio plasmids faciliate gene regulation and improve existing solutions like CRISPR-Cas9. CRISPR-Cas9 cuts DNA at a precise location, guided there by a sequence-specific RNA. dCas9 has been modified so that it no longer cuts DNA, but it can still be guided to specific sequences and will bind to them. dCas9 can be fused to protein tags or the effector domains of transcription factors (proteins that bind DNA in promoter regions and simultaneously bind other regulatory proteins via effector domains, which can activate or repress transcription and thereby affect gene expression) to develop customized DNA binding proteins. In comparison to common techniques like CRISPRa and CRISPRi, the casilio system offers major advantages in transcription regulation:

  • Higher specificity: PBS extends GuideRNA length thus reducing offsite effects.
  • Multiplexing: Enables new set of experiments through using different effector proteins simultaneously.
  • Time savings
  • Lower error rate
  • Lower risk of sample degradation
dCas9E1dCas9E2dCas9E3sgRNA1sgRNA2sgRNA3
Fig.2: Multiplexing Capabilities of the Casilio hybrid system.

12 results :

Casilio Regulation EpEd Mammalian Expression Vector pmax-mCBPHAT-4xNLS-PUFa-2xNLS Kanamycin CMV Promoter unconjugated Transient
Type:
Casilio Regulation
Application:
Epigenetic Editing
Gene:
mCBPHAT-4xNLS-PUFa-2xNLS
Species:
Various Species
Vector type:
Mammalian Expression Vector
Resistance Gene:
Kanamycin
Promoter:
CMV Promoter
Expression:
Transient
Catalog No. ABIN7529447
Casilio Regulation EpEd Mammalian Expression Vector pmax-dCas Kanamycin CMV Promoter unconjugated Transient
Type:
Casilio Regulation
Application:
Epigenetic Editing
Gene:
dCas9
Species:
Various Species
Vector type:
Mammalian Expression Vector
Vector backbone:
pmax-dCas
Resistance Gene:
Kanamycin
Promoter:
CMV Promoter
Expression:
Transient
Catalog No. ABIN7529448
Casilio Regulation EpEd Mammalian Expression Vector pmax-BFPKRAB-4xNLS-PUFa-2xNLS-3xFLAG Kanamycin CMV Promoter BFP Transient
Type:
Casilio Regulation
Application:
Epigenetic Editing
Gene:
BFPKRAB-4xNLS-PUFa-2xNLS-3xFLAG
Species:
Various Species
Vector type:
Mammalian Expression Vector
Resistance Gene:
Kanamycin
Promoter:
CMV Promoter
Fusion Tag:
BFP
Expression:
Transient
Catalog No. ABIN7529446
Casilio Regulation EpEd Mammalian Expression Vector pmax-3xFLAG-4xNLS-PUFc-2xNLS-p65HSF1 Kanamycin CMV Promoter unconjugated Transient
Type:
Casilio Regulation
Application:
Epigenetic Editing
Gene:
3xFLAG-4xNLS-PUFc-2xNLS-p65HSF1
Species:
Various Species
Vector type:
Mammalian Expression Vector
Resistance Gene:
Kanamycin
Promoter:
CMV Promoter
Expression:
Transient
Catalog No. ABIN7529445
Casilio Regulation EpEd Lentiviral Vector pLX-CMV_CAG-PUFa-hNEIL2-hTET1 Ampicillin CMV Promoter unconjugated Transient
Type:
Casilio Regulation
Application:
Epigenetic Editing
Gene:
PUFa-hNEIL2-hTET1
Species:
Various Species
Vector type:
Lentiviral Vector
Resistance Gene:
Ampicillin
Selection Marker:
Puromycin
Promoter:
CMV Promoter
Expression:
Transient
Catalog No. ABIN7529439
Casilio Regulation EpEd Lentiviral Vector pLX-CMV_CAG-PUFa-p65HSF1 Ampicillin CMV Promoter unconjugated Transient
Type:
Casilio Regulation
Application:
Epigenetic Editing
Gene:
PUFa-p65HSF1
Species:
Various Species
Vector type:
Lentiviral Vector
Vector backbone:
pLX-CMV_CAG-PUFa-p65HSF1
Resistance Gene:
Ampicillin
Selection Marker:
Puromycin
Promoter:
CMV Promoter
Expression:
Transient
Catalog No. ABIN7529440
Casilio Regulation EpEd Lentiviral Vector pLX-CMV_CAG-hGADD45A-PUFa-hTET1 Ampicillin CMV Promoter unconjugated Transient
Type:
Casilio Regulation
Application:
Epigenetic Editing
Gene:
HGADD45A-PUFa-hTET1
Species:
Various Species
Vector type:
Lentiviral Vector
Resistance Gene:
Ampicillin
Selection Marker:
Puromycin
Promoter:
CMV Promoter
Expression:
Transient
Catalog No. ABIN7529441
Casilio Regulation EpEd Lentiviral Vector pLX-CMV_CAG-PUFa-hTET1 Ampicillin CMV Promoter unconjugated Transient
Type:
Casilio Regulation
Application:
Epigenetic Editing
Gene:
PUFa-hTET1
Species:
Various Species
Vector type:
Lentiviral Vector
Vector backbone:
pLX-CMV_CAG-PUFa-hTET1
Resistance Gene:
Ampicillin
Selection Marker:
Puromycin
Promoter:
CMV Promoter
Expression:
Transient
Catalog No. ABIN7529442
Casilio Regulation EpEd Mammalian Expression Vector pmax-NLS-PUFa-VP64 Kanamycin CMV Promoter unconjugated Transient
Type:
Casilio Regulation
Application:
Epigenetic Editing
Gene:
NLS-PUFa-VP64
Species:
Various Species
Vector type:
Mammalian Expression Vector
Vector backbone:
pmax-NLS-PUFa-VP64
Resistance Gene:
Kanamycin
Promoter:
CMV Promoter
Expression:
Transient
Catalog No. ABIN7529443
Casilio Regulation EpEd Mammalian Expression Vector pmax-NLS-PUFc-VP64 Kanamycin CMV Promoter unconjugated Transient
Type:
Casilio Regulation
Application:
Epigenetic Editing
Gene:
NLS-PUFc-VP64
Species:
Various Species
Vector type:
Mammalian Expression Vector
Vector backbone:
pmax-NLS-PUFc-VP64
Resistance Gene:
Kanamycin
Promoter:
CMV Promoter
Expression:
Transient
Catalog No. ABIN7529444
Casilio Regulation EpEd Mammalian Expression Vector pX-SPFE-BbsI-sgRNA-5xPBSc Ampicillin U6 Promoter unconjugated Transient
Type:
Casilio Regulation
Application:
Epigenetic Editing
Gene:
SgRNA-5xPBSc
Species:
Various Species
Vector type:
Mammalian Expression Vector
Vector backbone:
pX-SPFE-BbsI-sgRNA-5xPBSc
Resistance Gene:
Ampicillin
Promoter:
U6 Promoter
Expression:
Transient
Catalog No. ABIN7529437
Casilio Regulation EpEd Mammalian Expression Vector pX-SPFE-BbsI-sgRNA-5xPBSa Ampicillin U6 Promoter unconjugated Transient
Type:
Casilio Regulation
Application:
Epigenetic Editing
Gene:
SgRNA-5xPBSc
Species:
Various Species
Vector type:
Mammalian Expression Vector
Vector backbone:
pX-SPFE-BbsI-sgRNA-5xPBSa
Resistance Gene:
Ampicillin
Promoter:
U6 Promoter
Expression:
Transient
Catalog No. ABIN7529438
  • Type
    • Plasmid
  • Supplier
    • antibodies-online
  • Target
    • SgRNA-5xPBSc
    • 3xFLAG-4xNLS-PUFc-2xNLS-p65HSF1
    • BFPKRAB-4xNLS-PUFa-2xNLS-3xFLAG
    • HGADD45A-PUFa-hTET1
    • NLS-PUFa-VP64
    • NLS-PUFc-VP64
    • PUFa-hNEIL2-hTET1
    • PUFa-hTET1
    • PUFa-p65HSF1
    • dCas9
    • mCBPHAT-4xNLS-PUFa-2xNLS
  • Reactivity
    • Various Species
  • Application
    • Epigenetic Editing (EpEd)
  • Conjugate
    • Un-conjugated
    • BFP
  • Vector Backbone
    • pLX-CMV_CAG-PUFa-hNEIL2-hTET1
    • pLX-CMV_CAG-PUFa-hTET1
    • pLX-CMV_CAG-PUFa-p65HSF1
    • pLX-CMV_CAG-hGADD45A-PUFa-hTET1
    • pX-SPFE-BbsI-sgRNA-5xPBSa
    • pX-SPFE-BbsI-sgRNA-5xPBSc
    • pmax-3xFLAG-4xNLS-PUFc-2xNLS-p65HSF1
    • pmax-BFPKRAB-4xNLS-PUFa-2xNLS-3xFLAG
    • pmax-NLS-PUFa-VP64
    • pmax-NLS-PUFc-VP64
    • pmax-dCas
    • pmax-mCBPHAT-4xNLS-PUFa-2xNLS
  • Vector
    • Mammalian Expression Vector
    • Lentiviral Vector
  • Insert Casilio Regulation
    • Casilio Regulation
    • cDNA
    • ORF
    • shRNA
    • gRNA + Cas9
    • gRNA
    • Empty
    • Casilio HDR
    • Casilio Imaging
  • Bacterial Resistance
    • Ampicillin
    • Kanamycin
  • Expression Type
    • Transient
  • Selectable Marker
    • Puromycin
  • Promoter
    • CMV Promoter
    • U6 Promoter
  • Format
    • Liquid
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