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Intres® Expression Vectors

Selectable markers are widely used in lentiviral transgenesis and CRISPR/Cas-mediated genome editing for selecting engineered cells with a desired genotype but the variety of markers is limited. Selectable markers work well for research involving single transgene, but adding more than one transgene to cells can be challenging to track. There are a limited number of fluorescence proteins, and subjecting cells to multiple antibiotic selections after each plasmid is delivered can harm them.

To address the issue, antibodies-online offers Intres Expression Vectors for split selectable markers (SSM). Intres stands for "Intein-split resistance", Intres Expression Vectors can indicate the successful integration of multiple transgenes. Intres Expression Vectors can be also used to facilitate genome engineering using the CRISPR/Cas system when it is used to “knock-in” (add) a genetic sequence at a specific location in the genome.

What are Split Selectable Markers?

SSM involve dividing the marker genes at specific sites and fusing them to protein splicing elements. The divided fragments are then added to separate plasmids, each of which also includes a transgene. When both plasmids are successfully delivered into a cell, a full-length marker protein is produced via protein trans-splicing. Cells that only receive one plasmid will not express the full marker protein and can therefore be selected out via antibiotic treatment or identified via lack of fluorescence. Cells receiving both plasmids could be selected at very high percentages, generally more than 90% and up to 100%, for subsequent transgenic research.

How are Selectable Markers Utilized?

Split selectable markers are created by dividing fluorescent proteins or antibiotic resistance proteins into several segments fused to protein splicing elements called “inteins” (fig.1). They can be separately co-segregated with different transgenic vectors, and rejoin via protein trans-splicing to reconstitute a full-length marker protein in host cells receiving all intended vectors.

Split selectable markers: Intein-Split Resistance (Intres) Expression Vectors

Fig.1: Split selectable marker can be used for co-selection of two separate transgenic vectors (table1). The coding sequence of a selectable marker is split into an N-terminal fragment (MarN) and a C-terminal fragment (kerC) and separately cloned upstream of an N-terminal fragment of a split intein (IntN) and downstream of a C-terminal fragment of the split intein (IntC), respectively, on two different vectors each carrying a different transgene. These vectors are delivered to cells yielding sub-populations of cells containing either one, or both of the vectors. Only cells with both vectors expressing the two intein-split selectable marker fragments (“markertrons”) undergo protein trans-splicing to reconstitute a full-length selectable marker, allowing specific selection and enrichment of the double transgenic cells.

What are the Advantages of Intres Expression Vectors

The system is not limited to simple two-plasmid applications, it is possible to select up to six transgenes using a single split selectable marker. In addition, split selectable markers can be used to facilitate genome engineering using the CRISPR/Cas system when it is used to “knock-in” (add) a genetic sequence at a specific location in the genome. Current precision editing and knock-in methods are inefficient, underscoring the need for effective marker and cell selection systems. Using split markers in concert with CRISPR/Cas editing again yielded very high percentages of cells with the desired genotypes following selection and growth.

Choose Intres Vectors Combinations

antibodies-online offers Intres Expression Vectors. Choose from vector combinations in the table to utilize your own inserts. Or discover all available split selectable marker vectors below.

Table. 1: Intres Expression Vectors Combination for SSM
ABIN Backbone Selection Marker
ABIN7539390 pLX-DEST-IRES-Hygro(1-89)-NpuDnaE(N) Hygromycin
ABIN7539391 pLX-DEST-IRES-NpuDnaE(C)-Hygro(90-341) Hygromycin
ABIN7539406 pLX-DEST-IRES-Neo(1-194)-NpuDnaE(N) Neomycin
ABIN7539407 pLX-DEST-IRES-NpuDnaE(C)-Neo(195-267) Neomycin
ABIN7539412 pLX-DEST-IRES-Puro(1-119)-NpuDnaE(N) Puromycin
ABIN7539413 pLX-DEST-IRES-NpuDnaE(C)-Puro(120-199) Puromycin

References

  1. Jillette, Du, Zhu, Cardoz, Cheng: "Split selectable markers." in: Nature communications, Vol. 10, Issue 1, pp. 4968, (2020) (PubMed).

Intres® is a registred trademark of the Jackson Laboratory

33 results :

Intres SSM Mammalian Expression Vector pLX-SspDnaB(C-S0)-Hygro(201-341)-IRES-mCherry Ampicillin CMV Promoter mCherry Transient
Type:
Intres
Application:
Split-Selectable Marker
Gene:
SspDnaB(C-S0)-Hygro(201-341)
Species:
Various Species
Vector type:
Mammalian Expression Vector
Resistance Gene:
Ampicillin
Promoter:
CMV Promoter
Fusion Tag:
mCherry
Expression:
Transient
Catalog No. ABIN7539388
Intres SSM Mammalian Expression Vector pLX-NpuDnaE(C)-Hygro(90-341)-IRES-mCherry Ampicillin CMV Promoter mCherry Transient
Type:
Intres
Application:
Split-Selectable Marker
Gene:
NpuDnaE(C)-Hygro(90-341)
Species:
Various Species
Vector type:
Mammalian Expression Vector
Resistance Gene:
Ampicillin
Promoter:
CMV Promoter
Fusion Tag:
mCherry
Expression:
Transient
Catalog No. ABIN7539389
Intres SSM Lentiviral Vector pLX-DEST-IRES-Hygro(1-89)-NpuDnaE(N) Ampicillin CMV Promoter unconjugated Transient
Type:
Intres
Application:
Split-Selectable Marker
Gene:
Hygro(1-89)-NpuDnaE(N)
Species:
Various Species
Vector type:
Lentiviral Vector
Resistance Gene:
Ampicillin
Promoter:
CMV Promoter
Expression:
Transient
Catalog No. ABIN7539390
Intres SSM Lentiviral Vector pLX-[TagBFP2]-IRES-Hygro(1-89)-NpuDnaE(N) Ampicillin CMV Promoter BFP Transient
Type:
Intres
Application:
Split-Selectable Marker
Gene:
Hygro(1-89)-NpuDnaE(N)
Species:
Various Species
Vector type:
Lentiviral Vector
Resistance Gene:
Ampicillin
Promoter:
CMV Promoter
Fusion Tag:
BFP
Expression:
Transient
Catalog No. ABIN7539392
Intres SSM Lentiviral Vector pLX-gp411(C)-Hygro(52-341)-IRES-mCherry Ampicillin CMV Promoter mCherry Transient
Type:
Intres
Application:
Split-Selectable Marker
Gene:
Gp411(C)-Hygro(52-341)
Species:
Various Species
Vector type:
Lentiviral Vector
Resistance Gene:
Ampicillin
Promoter:
CMV Promoter
Fusion Tag:
mCherry
Expression:
Transient
Catalog No. ABIN7539395
Intres SSM Lentiviral Vector pLX-Hygro(1-51)-gp411(N)-IRES-TagBFP2 Ampicillin CMV Promoter BFP Transient
Type:
Intres
Application:
Split-Selectable Marker
Gene:
Hygro(1-51)-Gp411(N)
Species:
Various Species
Vector type:
Lentiviral Vector
Resistance Gene:
Ampicillin
Promoter:
CMV Promoter
Fusion Tag:
BFP
Expression:
Transient
Catalog No. ABIN7539394
Intres SSM Mammalian Expression Vector pLX-NpuDnaE(C)-Hygro(90-200)-SspDnaB(N-S0)-IRES-EGFP Ampicillin CMV Promoter EGFP Transient
Type:
Intres
Application:
Split-Selectable Marker
Gene:
NpuDnaE(C)-Hygro(90-200)-SspDnaB(N-S0)
Species:
Various Species
Vector type:
Mammalian Expression Vector
Resistance Gene:
Ampicillin
Promoter:
CMV Promoter
Fusion Tag:
EGFP
Expression:
Transient
Catalog No. ABIN7539387
Intres SSM Lentiviral Vector pLX-[mCherry]-IRES-NpuDnaE(C)-Hygro(90-341) Ampicillin CMV Promoter mCherry Transient
Type:
Intres
Application:
Split-Selectable Marker
Gene:
NpuDnaE(C)-Hygro(90-341)
Species:
Various Species
Vector type:
Lentiviral Vector
Resistance Gene:
Ampicillin
Promoter:
CMV Promoter
Fusion Tag:
mCherry
Expression:
Transient
Catalog No. ABIN7539393
Intres SSM Lentiviral Vector pLX-Hygro(1-89)-NpuDnaE(N)-IRES-TagBFP2 Ampicillin CMV Promoter BFP Transient
Type:
Intres
Application:
Split-Selectable Marker
Gene:
Hygro(1-89)-NpuDnaE(N)
Species:
Various Species
Vector type:
Lentiviral Vector
Resistance Gene:
Ampicillin
Promoter:
CMV Promoter
Fusion Tag:
BFP
Expression:
Transient
Catalog No. ABIN7539386
Intres SSM Lentiviral Vector pLX-DEST-IRES-NpuDnaE(C)-Hygro(90-341) Ampicillin CMV Promoter unconjugated Transient
Type:
Intres
Application:
Split-Selectable Marker
Gene:
NpuDnaE(C)-Hygro(90-341)
Species:
Various Species
Vector type:
Lentiviral Vector
Resistance Gene:
Ampicillin
Promoter:
CMV Promoter
Expression:
Transient
Catalog No. ABIN7539391
Intres SSM Lentiviral Vector pLX-Hygro(1-130)-NrdJ1(N)-IRES-TagBFP2 Ampicillin CMV Promoter BFP Transient
Type:
Intres
Application:
Split-Selectable Marker
Gene:
Hygro(1-130)-NrdJ1(N)
Species:
Various Species
Vector type:
Lentiviral Vector
Resistance Gene:
Ampicillin
Promoter:
CMV Promoter
Fusion Tag:
BFP
Expression:
Transient
Catalog No. ABIN7539396
Intres SSM Lentiviral Vector pLX-gp411(C)-Hygro(52-89)-NpuDnaE(N)-IRES-GFP Ampicillin CMV Promoter EGFP Transient
Type:
Intres
Application:
Split-Selectable Marker
Gene:
Gp411(C)-Hygro(52-89)-NpuDnaE(N)
Species:
Various Species
Vector type:
Lentiviral Vector
Resistance Gene:
Ampicillin
Promoter:
CMV Promoter
Fusion Tag:
EGFP
Expression:
Transient
Catalog No. ABIN7539400
Intres SSM Lentiviral Vector pLX-Hygro(1-258)-IMPDH1(N)-IRES-TagBFP2 Ampicillin CMV Promoter BFP Transient
Type:
Intres
Application:
Split-Selectable Marker
Gene:
Hygro(1-258)-IMPDH1(N)
Species:
Various Species
Vector type:
Lentiviral Vector
Resistance Gene:
Ampicillin
Promoter:
CMV Promoter
Fusion Tag:
BFP
Expression:
Transient
Catalog No. ABIN7539398
Intres SSM Lentiviral Vector pLX-NrdJ1(C)-Hygro(131-341)-IRES-mCherry Ampicillin CMV Promoter mCherry Transient
Type:
Intres
Application:
Split-Selectable Marker
Gene:
NrdJ1(C)-Hygro(131-341)
Species:
Various Species
Vector type:
Lentiviral Vector
Resistance Gene:
Ampicillin
Promoter:
CMV Promoter
Fusion Tag:
mCherry
Expression:
Transient
Catalog No. ABIN7539397
Intres SSM Lentiviral Vector pLX-IMPDH1(C)-Hygro(259-341)-IRES-mCherry Ampicillin CMV Promoter mCherry Transient
Type:
Intres
Application:
Split-Selectable Marker
Gene:
IMPDH1(C)-Hygro(259-341)
Species:
Various Species
Vector type:
Lentiviral Vector
Resistance Gene:
Ampicillin
Promoter:
CMV Promoter
Fusion Tag:
mCherry
Expression:
Transient
Catalog No. ABIN7539399
Intres SSM Lentiviral Vector pLX-SspDnaB(C)-Hygro(201-258)-IMPDH1(N)-IRES-GFP Ampicillin CMV Promoter EGFP Transient
Type:
Intres
Application:
Split-Selectable Marker
Gene:
SspDnaB(C)-Hygro(201-258)-IMPDH1(N)
Species:
Various Species
Vector type:
Lentiviral Vector
Resistance Gene:
Ampicillin
Promoter:
CMV Promoter
Fusion Tag:
EGFP
Expression:
Transient
Catalog No. ABIN7539403
Intres SSM Lentiviral Vector pLX-NpuDnaE(C)-Neo(195-267)-IRES-mCherry Ampicillin CMV Promoter mCherry Transient
Type:
Intres
Application:
Split-Selectable Marker
Gene:
NpuDnaE(C)-Neo(195-267)
Species:
Various Species
Vector type:
Lentiviral Vector
Resistance Gene:
Ampicillin
Promoter:
CMV Promoter
Fusion Tag:
mCherry
Expression:
Transient
Catalog No. ABIN7539405
Intres SSM Lentiviral Vector pLX-Neo(1-194)-NpuDnaE(N)-IRES-TagBFP2 Ampicillin CMV Promoter BFP Transient
Type:
Intres
Application:
Split-Selectable Marker
Gene:
Neo(1-194)-NpuDnaE(N)
Species:
Various Species
Vector type:
Lentiviral Vector
Resistance Gene:
Ampicillin
Promoter:
CMV Promoter
Fusion Tag:
BFP
Expression:
Transient
Catalog No. ABIN7539404
Intres SSM Lentiviral Vector pLX-NrdJ1(C)-Hygro(131-200)-SspDnaB(N)-IRES-GFP Ampicillin CMV Promoter EGFP Transient
Type:
Intres
Application:
Split-Selectable Marker
Gene:
NrdJ1(C)-Hygro(131-200)-SspDnaB(N)
Species:
Various Species
Vector type:
Lentiviral Vector
Resistance Gene:
Ampicillin
Promoter:
CMV Promoter
Fusion Tag:
EGFP
Expression:
Transient
Catalog No. ABIN7539402
Intres SSM Lentiviral Vector pLX-Puro(1-119)-NpuDnaE(N)-IRES-TagBFP2 Ampicillin CMV Promoter BFP Transient
Type:
Intres
Application:
Split-Selectable Marker
Gene:
Puro(1-119)-NpuDnaE(N)
Species:
Various Species
Vector type:
Lentiviral Vector
Resistance Gene:
Ampicillin
Promoter:
CMV Promoter
Fusion Tag:
BFP
Expression:
Transient
Catalog No. ABIN7539410
  • Type
    • Plasmid
  • Supplier
    • antibodies-online
  • Target
    • Hygro(1-89)-NpuDnaE(N)
    • Neo(1-194)-NpuDnaE(N)
    • NpuDnaE(C)-Hygro(90-341)
    • NpuDnaE(C)-Neo(195-267)
    • Puro(1-119)-NpuDnaE(N)
    • NpuDnaE(C)-Puro(120-199)
    • Blast(1-102)-NpuDnaE(N)
    • Gp411(C)-Hygro(52-341)
    • Gp411(C)-Hygro(52-89)-NpuDnaE(N)
    • Hygro(1-130)-NrdJ1(N)
    • Hygro(1-258)-IMPDH1(N)
    • Hygro(1-51)-Gp411(N)
    • IMPDH1(C)-Hygro(259-341)
    • NpuDnaE(C)-Blast(103-140)
    • NpuDnaE(C)-Hygro(90-130)-NrdJ1(N)
    • NpuDnaE(C)-Hygro(90-200)-SspDnaB(N-S0)
    • NrdJ1(C)-Hygro(131-200)-SspDnaB(N)
    • NrdJ1(C)-Hygro(131-341)
    • Puro(1-98)-NrdJ1(N)
    • SspDnaB(C)-Hygro(201-258)-IMPDH1(N)
    • SspDnaB(C-S0)-Hygro(201-341)
    • iFP1.4-IRES-NrdJ1(C)-Puro(99-199)
  • Reactivity
    • Various Species
  • Application
    • Split-Selectable Marker (SSM)
  • Conjugate
    • BFP
    • mCherry
    • EGFP
    • Un-conjugated
    • iFP1.4
  • Vector Backbone
    • pLX-Blast(1-102)-NpuDnaE(N)-IRES-TagBFP2
    • pLX-DEST-IRES-Hygro(1-89)-NpuDnaE(N)
    • pLX-DEST-IRES-Neo(1-194)-NpuDnaE(N)
    • pLX-DEST-IRES-NpuDnaE(C)-Hygro(90-341)
    • pLX-DEST-IRES-NpuDnaE(C)-Neo(195-267)
    • pLX-DEST-IRES-NpuDnaE(C)-Puro(120-199)
    • pLX-DEST-IRES-Puro(1-119)-NpuDnaE(N)
    • pLX-Hygro(1-130)-NrdJ1(N)-IRES-TagBFP2
    • pLX-Hygro(1-258)-IMPDH1(N)-IRES-TagBFP2
    • pLX-Hygro(1-51)-gp411(N)-IRES-TagBFP2
    • pLX-Hygro(1-89)-NpuDnaE(N)-IRES-TagBFP2
    • pLX-IMPDH1(C)-Hygro(259-341)-IRES-mCherry
    • pLX-NLS-GFP-IRES-Puro(1-98)-NrdJ1(N)
    • pLX-Neo(1-194)-NpuDnaE(N)-IRES-TagBFP2
    • pLX-NpuDnaE(C)-Blast(103-140)-IRES-mCherry
    • pLX-NpuDnaE(C)-Hygro(90-130)-NrdJ1(N)-IRES-GFP
    • pLX-NpuDnaE(C)-Hygro(90-200)-SspDnaB(N-S0)-IRES-EGFP
    • pLX-NpuDnaE(C)-Hygro(90-341)-IRES-mCherry
    • pLX-NpuDnaE(C)-Neo(195-267)-IRES-mCherry
    • pLX-NrdJ1(C)-Hygro(131-200)-SspDnaB(N)-IRES-GFP
    • pLX-NrdJ1(C)-Hygro(131-341)-IRES-mCherry
    • pLX-Puro(1-119)-NpuDnaE(N)-IRES-TagBFP2
    • pLX-SspDnaB(C)-Hygro(201-258)-IMPDH1(N)-IRES-GFP
    • pLX-SspDnaB(C-S0)-Hygro(201-341)-IRES-mCherry
    • pLX-[TagBFP2]-IRES-Hygro(1-89)-NpuDnaE(N)
    • pLX-[TagBFP2]-IRES-Neo(1-194)-NpuDnaE(N)
    • pLX-[TagBFP2]-IRES-Puro(1-119)-NpuDnaE(N)
    • pLX-[mCherry]-IRES-NpuDnaE(C)-Hygro(90-341)
    • pLX-[mCherry]-IRES-NpuDnaE(C)-Neo(195-267)
    • pLX-[mCherry]-IRES-NpuDnaE(C)-Puro(120-199)
    • pLX-gp411(C)-Hygro(52-341)-IRES-mCherry
    • pLX-gp411(C)-Hygro(52-89)-NpuDnaE(N)-IRES-GFP
    • pLX-iFP1.4-IRES-NrdJ1(C)-Puro(99-199)
  • Vector
    • Lentiviral Vector
    • Mammalian Expression Vector
  • Insert Intres
    • Intres
    • cDNA
    • ORF
    • shRNA
    • gRNA + Cas9
    • gRNA
    • Empty
    • Casilio Regulation
    • Casilio HDR
    • Casilio Imaging
  • Bacterial Resistance
    • Ampicillin
    • Chloramphenicol
  • Expression Type
    • Transient
  • Promoter
    • CMV Promoter
  • Format
    • Liquid
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